A Comparison of Human and Bovine Escherichia coli O157:H7 Isolates by Toxin Genotype, Plasmid Profile, and Bacteriophage -Restriction Fragment Length Polymorphism Profile
| Autor: | Michael Paros, Phillip I. Tarr, Dale D. Hancock, Harold H. Kim, Thomas E. Besser |
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| Rok vydání: | 1993 |
| Předmět: |
Bacterial Toxins
Shiga Toxin 1 medicine.disease_cause Shiga Toxin 2 Restriction fragment Microbiology Bacteriophage Plasmid Genotype Escherichia coli medicine Animals Humans Immunology and Allergy Escherichia coli Infections Disease Reservoirs Genetics biology Toxin biology.organism_classification Bacteriophage lambda Enterobacteriaceae Infectious Diseases biology.protein Cattle Restriction fragment length polymorphism Plasmids |
| Zdroj: | Journal of Infectious Diseases. 168:1300-1303 |
| ISSN: | 1537-6613 0022-1899 |
| Popis: | Foods of bovine origin have been linked to human disease outbreaks caused by Escherichia coli O157:H7 and may be linked to the more common sporadic cases as well. In this study, E. coli O157:H7 from the bovine reservoir (22 isolates: 12 from dairy and 10 from beef breed cows) and from human patients (50 isolates from sporadic human infections) were compared using Shiga-like toxin genotypes, plasmid profiles, and DNA restriction fragment length polymorphisms identified with a bacteriophage lambda probe (lambda-RFLP). Twenty-three lambda-RFLP profiles, 4 Shiga-like toxin genotypes, and 8 plasmid profiles were identified among the isolates tested. Together the typing methods distinguished 43 strains, of which 3 were isolated from both humans (5 isolates) and cattle (6 isolates; 5 from dairy herds). These data demonstrate the value of lambda-RFLP as a means of strain identification for E. coli O157:H7. |
| Databáze: | OpenAIRE |
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