Hypoxia-inducible factor-1α and Wnt/β-catenin signaling pathways promote the invasion of hypoxic gastric cancer cells
| Autor: | Hong‑Lan Liu, Jun‑Wen Zhang, Guang‑Rong Ding, Peng‑Fei Liao, Dang Liu |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Cancer Research
Beta-catenin Mice Nude matrix metalloproteinase-7 Biochemistry Mice Stomach Neoplasms Cell Line Tumor Genetics medicine Animals Humans Neoplasm Invasiveness Hypoxia Molecular Biology Wnt Signaling Pathway beta Catenin Gene knockdown Mice Inbred BALB C Oncogene biology hypoxia-inducible factor-1α microRNA urokinase-type plasminogen activator Stomach Wnt signaling pathway Articles Hypoxia (medical) β-catenin Hypoxia-Inducible Factor 1 alpha Subunit Molecular biology Cell Hypoxia Oncology Hypoxia-inducible factors Apoptosis Gastric Mucosa Cancer cell biology.protein Cancer research Molecular Medicine medicine.symptom |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 |
| Popis: | The present study aimed to examine the association between hypoxia-inducible factor (HIF)-1α and the Wnt/β-catenin signaling pathway in a hypoxic environment. The study also aimed to explore the possible mechanisms underlying the invasion of hypoxic gastric cancer cells in vitro and in vivo. The pcDNA™ 6.2-GW/EmGFP-miR-β-catenin plasmid was transfected into SGC-7901 gastric cancer cells, resulting in cells with stable suppression of β-catenin expression. The biological characteristics of the control, liposome, negative control, β-catenin knockdown, hypoxia and hypoxia β-catenin knockdown groups were tested using an invasion assay. The differences in the invasive capacity of the control, negative control and liposome groups were not statistically significant. However, the hypoxia group demonstrated a significantly enhanced invasive capacity, as compared with that in the control group (P |
| Databáze: | OpenAIRE |
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