A β-Catenin-Independent Dorsalization Pathway Activated by Axin/JNK Signaling and Antagonized by Aida
| Autor: | Zhiyun Ye, Bo Xiong, Zhen Xu, Shu-Yong Lin, Hai-Meng Zhou, Anming Meng, Ying Cao, Sheng-Cai Lin, Min Zhang, Jiahuai Han, Ying Han, Siu Chiu Chan, Zailian Lu, Wen Luo, Yanning Rui |
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| Rok vydání: | 2007 |
| Předmět: |
Scaffold protein
Embryo Nonmammalian MAP Kinase Signaling System macromolecular substances Biology General Biochemistry Genetics and Molecular Biology Cell Line Mice Axin Protein In vivo Chlorocebus aethiops Animals Humans Molecular Biology Zebrafish beta Catenin Body Patterning Zygote Embryogenesis JNK Mitogen-Activated Protein Kinases Wnt signaling pathway Embryo Cell Biology Oligonucleotides Antisense Zebrafish Proteins Cell biology Enzyme Activation Repressor Proteins Wnt Proteins Phenotype Catenin COS Cells Zebrafish embryo Carrier Proteins Dimerization Protein Binding Developmental Biology |
| Zdroj: | Developmental Cell. 13(2):268-282 |
| ISSN: | 1534-5807 |
| DOI: | 10.1016/j.devcel.2007.07.006 |
| Popis: | Axin is a scaffold protein that controls multiple important pathways, including the canonical Wnt pathway and JNK signaling. Here we have identified an Axin-interacting protein, Aida, which blocks Axin-mediated JNK activation by disrupting Axin homodimerization. During investigation of in vivo functions of Axin/JNK signaling and aida in development, it was found that Axin, besides ventralizing activity by facilitating beta-catenin degradation, possesses a dorsalizing activity that is mediated by Axin-induced JNK activation. This dorsalizing activity is repressed when aida is overexpressed in zebrafish embryos. Whereas Aida-MO injection leads to dorsalized embryos, JNK-MO and MKK4-MO can ventralize embryos. The anti-dorsalization activity of aida is conferred by its ability to block Axin-mediated JNK activity. We further demonstrate that dorsoventral patterning regulated by Axin/JNK signaling is independent of maternal or zygotic Wnt signaling. We have thus identified a dorsalization pathway that is exerted by Axin/JNK signaling and its inhibitor Aida during vertebrate embryogenesis. |
| Databáze: | OpenAIRE |
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