DNA binding, photoactivated DNA cleavage and cytotoxic activity of Cu(II) and Co(II) based Schiff-base azo photosensitizers
| Autor: | Atanu Barik, K. Indira Priyadarsini, H. S. Bhojya Naik, B. Vinay Kumar, S.M. Pradeepa, M. C. Prabhakara |
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| Rok vydání: | 2014 |
| Předmět: |
Spectrophotometry
Infrared Stereochemistry Carboxylic acid Proton Magnetic Resonance Spectroscopy Quantum yield Electrons Nucleic Acid Denaturation Mass Spectrometry Analytical Chemistry chemistry.chemical_compound Inhibitory Concentration 50 Animals Humans Photosensitizer DNA Cleavage Instrumentation Spectroscopy Schiff Bases Benzoic acid chemistry.chemical_classification Electrophoresis Agar Gel Schiff base Photosensitizing Agents Singlet Oxygen Ligand Viscosity Cobalt DNA Atomic and Molecular Physics and Optics chemistry Solubility MCF-7 Cells Cattle Spectrophotometry Ultraviolet pUC19 Copper |
| Zdroj: | Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 141 |
| ISSN: | 1873-3557 |
| Popis: | A new class of Cu(II) and Co(II) complexes of azo-containing Schiff base of the type [Cu(L1)2] and [Co(L1)2], where L1 = 4-[(E)-{2-hydroxy-3-[(E)-(4-bromophenyl)diazenyl]benzylidene}amino]benzoic acid have been synthesized and characterized. Extension of conjugation and the presence of free carboxylic acid group of the ligand L1 increased the wavelength of the complexes from visible region to the near IR region (620–850 nm). The Cu(II) and Co(II) complexes interacted with CT-DNA via intercalative mode with the respective Kb value of 3.2 × 104 M−1 and 2.9 × 104 M−1 and acted as proficient photocleavers of SC pUC19 DNA in UV-A light, forming 1O2 as the reactive oxygen species with the quantum yield of 0.38 and 0.36, respectively. Furthermore, the Cu(II) and Co(II) complexes showed photocytotoxicity toward two selected tumor cell lines MCF-7 and A549 by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) method, and the Cu(II) complex exhibits higher photocytotoxicity than Co(II) complex against each of the selected cell lines, this result is identical with their DNA binding ability order. |
| Databáze: | OpenAIRE |
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