RNAi Screen Identifies MTA1 as an Epigenetic Modifier of Differentiation Commitment in Human HSPCs

Autor:	Kristijonas Žemaitis, Agatheeswaran Subramaniam, Roman Galeev, Aurel Prosz, Maria Jassinskaja, Jenny Hansson, Jonas Larsson
Rok vydání:	2022
Předmět:	Cancer Research Antigens CD34 Cell Differentiation Cell Biology Hematology Fetal Blood Hematopoietic Stem Cells Repressor Proteins Mice Trans-Activators Genetics Humans Animals RNA Interference Molecular Biology
Zdroj:	Experimental Hematology. 115:20-29
ISSN:	0301-472X
Popis:	The molecular mechanisms regulating key fate decisions of hematopoietic stem cells (HSCs) remain incompletely understood. Here, we targeted global shRNA libraries to primary human hematopoietic stem and progenitor cells (HSPCs) to screen for modifiers of self-renewal and differentiation, and identified metastasis-associated 1 (MTA1) as a negative regulator of human HSPC propagation in vitro. Knockdown of MTA1 by independent shRNAs in primary human cord blood (CB) HSPCs led to a cell expansion during culture and a relative accumulation of immature CD34
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7571f264b952698eb98afd27c5e0563d https://doi.org/10.1016/j.exphem.2022.08.004 Zobrazit plný text záznamu Full Text from ScienceDirect