Autor: |
Kristijonas Žemaitis, Agatheeswaran Subramaniam, Roman Galeev, Aurel Prosz, Maria Jassinskaja, Jenny Hansson, Jonas Larsson |
Rok vydání: |
2022 |
Předmět: |
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Zdroj: |
Experimental Hematology. 115:20-29 |
ISSN: |
0301-472X |
Popis: |
The molecular mechanisms regulating key fate decisions of hematopoietic stem cells (HSCs) remain incompletely understood. Here, we targeted global shRNA libraries to primary human hematopoietic stem and progenitor cells (HSPCs) to screen for modifiers of self-renewal and differentiation, and identified metastasis-associated 1 (MTA1) as a negative regulator of human HSPC propagation in vitro. Knockdown of MTA1 by independent shRNAs in primary human cord blood (CB) HSPCs led to a cell expansion during culture and a relative accumulation of immature CD34 |
Databáze: |
OpenAIRE |
Externí odkaz: |
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