Human umbilical cord mesenchymal stromal cells as an adjunct therapy with therapeutic hypothermia in a piglet model of perinatal asphyxia

Autor: Xenia Charalambous, Tatenda Mutshiya, Kathryn A. Martinello, B. Weil, Mariya Hristova, Boris W. Kramer, Ingran Lingam, Mark W. Lowdell, Tiziana Boggini, Qin Yang, Nicola J. Robertson, Adnan Avdic-Belltheus, Alan Bainbridge, Christopher Meehan, Magdalena Sokolska, Xavier Golay
Přispěvatelé: RS: GROW - R4 - Reproductive and Perinatal Medicine, RS: MHeNs - R3 - Neuroscience, Kindergeneeskunde, MUMC+: MA Medische Staf Kindergeneeskunde (9)
Rok vydání: 2020
Předmět:
CEREBRAL ENERGY FAILURE
0301 basic medicine
Cancer Research
Swine
INFANTS
Umbilical cord
Umbilical Cord
chemistry.chemical_compound
0302 clinical medicine
Hypothermia
Induced
Immunology and Allergy
Hippocampus (mythology)
BRAIN
Genetics (clinical)
TUNEL assay
Full-Length Article
ENCEPHALOPATHY
BIRTH ASPHYXIA
medicine.anatomical_structure
Oncology
030220 oncology & carcinogenesis
GROWTH
HYPOXIA-ISCHEMIA
medicine.symptom
STEM-CELLS
STROKE
medicine.medical_specialty
Immunology
Phosphocreatine
03 medical and health sciences
Asphyxia
Internal medicine
Translational Research
medicine
Animals
Humans
Transplantation
business.industry
Mesenchymal Stem Cells
Cell Biology
Hypothermia
medicine.disease
Perinatal asphyxia
Disease Models
Animal
030104 developmental biology
Endocrinology
chemistry
Terminal deoxynucleotidyl transferase
Animals
Newborn
business
Zdroj: Cytotherapy
Cytotherapy, 23(6), 521-535. ELSEVIER SCI LTD
ISSN: 1477-2566
1465-3249
Popis: Background: With therapeutic hypothermia (HT) for neonatal encephalopathy, disability rates are reduced, but not all babies benefit. Pre-clinical rodent studies suggest mesenchymal stromal cells (MSCs) augment HT protection. Aims: The authors studied the efficacy of intravenous (IV) or intranasal (IN) human umbilical cord derived MSCs (huMSCs) as adjunct therapy to HT in a piglet model. Methods: A total of 17 newborn piglets underwent transient cerebral hypoxia-ischemia (HI) and were then randomized to (i) HT at 33.5 degrees C 1-13 h after HI (n = 7), (ii) HT+IV huMSCs (30 x 10(6) cells) at 24 h and 48 h after HI (n = 5) or (iii) HT+IN huMSCs (30 x 10(6) cells) at 24 h and 48 h after HI (n = 5). Phosphorus-31 and hydrogen-1 magnetic resonance spectroscopy (MRS) was performed at 30 h and 72 h and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-positive cells and oligodendrocytes quantified. In two further piglets, 30 x 10(6) IN PKH-labeled huMSCs were administered. Results: HI severity was similar between groups. Amplitude-integrated electroencephalogram (aEEG) recovery was more rapid for HT+IN huMSCs compared with HT from 25 h to 42 h and 49 h to 54 h (P
Databáze: OpenAIRE
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