Tomato seeds pretreated with Antifreeze protein type I (AFP I) promotes the germination under cold stress by regulating the genes involved in germination process
| Autor: | Swum Yi Kyu, Kyeung Il Park, Chang Kil Kim, Phyo Phyo Win Pe, Aung Htay Naing |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Transcription Genetic Short Communication Germination Plant Science Biology 01 natural sciences 03 medical and health sciences Solanum lycopersicum Antifreeze protein Gene Expression Regulation Plant Antifreeze Proteins Gene expression Animals RNA Messenger Gene Cold stress Plant Proteins Cold-Shock Response Fishes food and beverages Temperature stress digestive system diseases Proton-Translocating ATPases 030104 developmental biology Biochemistry Seedlings Seeds 010606 plant biology & botany |
| Zdroj: | Plant Signal Behav |
| Popis: | This study was conducted to investigate the involvement of antifreeze proteins (AFPs; type I and III) in the germination mechanism of tomato seeds under low temperature stress. Germination of the seeds grown at a room temperature (25°C) was observed on 5 days after sowing (DAS), while all seeds exposed to a low temperature started to germinate at 16 days after sowing (DAS). However, in comparison with control seeds (0 µg/l), seeds treated with AFP I (100, 300, or 500 µg/l) germinated earlier and at a higher percentage until 20 DAS, and seeds treated with 100 µg/l AFP I showed the highest percentage of germination. Surprisingly, AFP III did not significantly increase germination, and the rate was lower among 500 µg/l AFP III-treated seeds compared with control seeds (0 µg/l). The transcription levels of the plasma membrane-associated H+-ATPase gene and antioxidant-related superoxide dismutase (SOD) and catalase 1 (CAT1) genes were analyzed, and the transcription levels of the genes in the seeds grown at 25°C were relatively low. For low temperature-treated seeds, H+-ATPase in control seeds (0 µg/l) was higher compared with that in AFP I-treated seeds and was lower compared with that in AFP III-treated seeds. The expression levels of the antioxidant-related genes (SOD and CAT1) were lower in AFP I-treated seeds than in control seeds (0 µg/l); however, they were higher in AFP III-treated seeds than in control seeds (0 µg/l). Overall, compared with AFP III, AFP I may potentially function as a cold-protective agent by modulating the genes associated with seed germination. |
| Databáze: | OpenAIRE |
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