Characterization of bacterial strains capable of desulphurisation in soil and sediment samples from Antarctica
| Autor: | Gabriela Frois Duarte, Victor Satler Pylro, Douglas Boniek, Débora Figueiredo |
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| Rok vydání: | 2010 |
| Předmět: |
DNA
Bacterial inorganic chemicals Fossil Fuels Geologic Sediments Genotype Microorganism Colony Count Microbial Bulk soil Antarctic Regions Polycyclic aromatic hydrocarbon Thiophenes Microbiology Microbial ecology Botany Soil Pollutants Extreme environment Phylogeny Soil Microbiology chemistry.chemical_classification Rhizosphere Bacteria biology Biphenyl Compounds digestive oral and skin physiology Pseudomonas Sequence Analysis DNA General Medicine biology.organism_classification Adaptation Physiological respiratory tract diseases Kinetics Phenotype chemistry Environmental chemistry Molecular Medicine Sulfur Water Pollutants Chemical |
| Zdroj: | Extremophiles. 14:475-481 |
| ISSN: | 1433-4909 1431-0651 |
| DOI: | 10.1007/s00792-010-0326-3 |
| Popis: | The presence of sulphur in fossil fuels and the natural environment justifies the study of sulphur-utilising bacterial species and genes involved in the biodesulphurisation process. Technology has been developed based on the natural ability of microorganisms to remove sulphur from polycyclic aromatic hydrocarbon chains. This biotechnology aims to minimise the emission of sulphur oxides into the atmosphere during combustion and prevent the formation of acid rain. In this study, the isolation and characterization of desulphurising microorganisms in rhizosphere and bulk soil samples from Antarctica that were either contaminated with oil or uncontaminated was described. The growth of selected isolates and their capacity to utilise sulphur based on the formation of the terminal product of desulphurisation via the 4S pathway, 2-hydroxybiphenyl, was analysed. DNA was extracted from the isolates and BOX-PCR and DNA sequencing were performed to obtain a genomic diversity profile of cultivable desulphurising bacterial species. Fifty isolates were obtained showing the ability of utilising dibenzothiophene as a substrate and sulphur source for maintenance and growth when plated on selective media. However, only seven genetically diverse isolates tested positive for sulphur removal using the Gibbs assay. DNA sequencing revealed that these isolates were related to the genera Acinetobacter and Pseudomonas. |
| Databáze: | OpenAIRE |
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