Effects of dietary fat source and subtherapeutic levels of antibiotic on the bacterial community in the ileum of broiler chickens at various ages
| Autor: | Gerald W. Tannock, Ricarda M. Engberg, Ane Knarreborg, M.A. Simon, Bent Borg Jensen |
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| Rok vydání: | 2002 |
| Předmět: |
medicine.drug_class
Antibiotics Colony Count Microbial Ileum Biology medicine.disease_cause Applied Microbiology and Biotechnology Polymerase Chain Reaction Microbiology Microbial Ecology chemistry.chemical_compound Tallow medicine Animals Food science Salinomycin Ecology Bacteria Broiler Age Factors food and beverages Clostridium perfringens Dietary Fats Anti-Bacterial Agents medicine.anatomical_structure chemistry Composition (visual arts) Electrophoresis Polyacrylamide Gel Chickens Temperature gradient gel electrophoresis Food Science Biotechnology |
| Zdroj: | Knarreborg, A, Simon, M A, Engberg, R M, Jensen, B B & Tannock, G W 2002, ' Effects of dietary fat source and subtherapeutic levels of antibiotic on the bacterial community in the ileum of broiler chickens at various ages ', Applied and Environmental Microbiology, vol. 68, pp. 5918-5924 . |
| ISSN: | 0099-2240 |
| Popis: | The effect of dietary fat source (soy oil or a mixture of lard and tallow) and dietary supplementation with antibiotics (a combination of avilamycin at 10 mg kg of feed −1 and salinomycin at 40 mg kg of feed −1 ) on the bacterial community in the ileum of broiler chickens at different ages (7, 14, 21, and 35 days) was studied using PCR with denaturing gradient gel electrophoresis (DGGE) analysis and bacteriological culture. The bacterial origin of fragments in DGGE profiles was identified by sequencing. Bacterial enumeration results, together with PCR-DGGE profiles, showed that the composition of the microflora was age dependent and influenced by dietary fat source and antibiotic supplementation. An increased incidence of streptococci, enterobacteria, and Clostridium perfringens with age of the chickens was demonstrated. Lactobacilli and C. perfringens were the bacterial groups most strongly affected by the dietary treatments. Moreover, different strains (clonal variants of the alpha-toxin gene) of C. perfringens type A were detected in response to age, dietary fat source, and dietary supplementation with antibiotics. |
| Databáze: | OpenAIRE |
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