Ulvan from Ulva armoricana (Chlorophyta) activates the PI3K/Akt signalling pathway via TLR4 to induce intestinal cytokine production
| Autor: | Matthieu Le Goff, Hervé Demais, Michel Olivier, Sébastien Holbert, Mustapha Berri, Joëlle Dupont, Pi Nyvall Collén |
|---|---|
| Přispěvatelé: | Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Biovet Conseil, Pôle biotechnologique, Amadéite SAS, BPI-France/ISI Ulvans project (n°: I1110001W) to Amadéite company, Bréhan (France), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Olmix Group, ProdInra, Migration |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT] [SDV.BA] Life Sciences [q-bio]/Animal biology Algae medicine.medical_treatment Epithelial cells Biology 01 natural sciences Microbiology Immunostimulatory 03 medical and health sciences Immune system Western blot medicine Protein kinase B PI3K/AKT/mTOR pathway ComputingMilieux_MISCELLANEOUS medicine.diagnostic_test 010405 organic chemistry [SDV.BA]Life Sciences [q-bio]/Animal biology HEK 293 cells Ulvan 0104 chemical sciences 3. Good health Cell biology 030104 developmental biology Cytokine Intestinal Cell culture Phosphorylation Cytokines [SDV.IMM]Life Sciences [q-bio]/Immunology Agronomy and Crop Science |
| Zdroj: | Algal Research-Biomass, Biofuels and Bioproducts Algal Research-Biomass, Biofuels and Bioproducts, Elsevier, 2017, 28, pp.39-47. ⟨10.1016/j.algal.2017.10.008⟩ 9. European Symposium of Porcine Health Management (ESPHM 2017) 9. European Symposium of Porcine Health Management (ESPHM 2017), May 2017, Prague, Czech Republic. 2017 |
| ISSN: | 2211-9264 |
| DOI: | 10.1016/j.algal.2017.10.008⟩ |
| Popis: | The authors wish to thank Christelle Gouin (Amadeite) for the production of the demineralized algal extracts and for her contribution to the proximate analyses and Dr. Nathalie Guriec (CHU Brest) for the LPS analysis. We also gratefully acknowledge Dr. Isabelle Oswald (Toxalim Unit, INRA center of Toulouse, France) for kindly providing the IPEC-1 cell line.; International audience; The biological activities of water-soluble sulfated polysaccharides of green algae (ulvans) have been explored for use as bioactive molecules for the benefit of human and animal health. A purified ulvan fraction was prepared from the green algae Ulva armoricana harvested in the Brittany (France) and tested for its capacity to stimulate the immune response of the gut using an in vitro system of porcine intestinal epithelial (IPEC-1) cells. RT-qPCR and ELISA analyses showed a significant increase in the mRNA and protein expression of cytokines such as CCL20, IL8, and TNFα. Using human embryonic kidney (HEK) 293 reporter cell lines for pattern recognition receptors, ulvan was found to primarily stimulate TLR4. We also examined the effect of the ulvan fraction on different signalling pathways involved in the activation of cytokine gene expression. Western blot analyses of ulvan-treated HEK293-TLR4 cells showed an increase in the phosphorylation of Akt and the p65 subunit of nuclear factor-κB. Inhibition of Akt phosphorylation with the specific inhibitor abrogated the ulvan-mediated enhancement of IL-8 secretion. The overall results showed that ulvan is an immunostimulatory compound by itself, and furthermore, it could be used to effectively complex and deliver TLR ligands to relevant immune cells in vaccination strategies. |
| Databáze: | OpenAIRE |
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