Adoptive transfer of NK 1.1+ lymphocytes in immune-mediated colitis: a pro-inflammatory or a tolerizing subgroup of cells?
| Autor: | Yoram Menachem, Yaron Ilan, Olga Kolker, Oren Shibolet, Ruslana Alper, Shivti Trop, Arnon Nagler |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Adoptive cell transfer Colon Lymphocyte Immunology Biology Microbiology Immune tolerance Natural killer cell Mice Immune system T-Lymphocyte Subsets Immune Tolerance medicine Animals Antigens Ly Lectins C-Type Antigens Colitis Peripheral tolerance induction Proteins hemic and immune systems medicine.disease Adoptive Transfer Mice Inbred C57BL Infectious Diseases medicine.anatomical_structure Antigens Surface Tumor necrosis factor alpha Spleen NK Cell Lectin-Like Receptor Subfamily B |
| Zdroj: | Microbes and Infection. 7:825-835 |
| ISSN: | 1286-4579 |
| DOI: | 10.1016/j.micinf.2005.03.019 |
| Popis: | T lymphocytes expressing NK1.1 marker (NKT) have been suggested to play crucial roles in immune modulation. Aim.– To determine the role of NK1.1+ cells in induction and maintenance of pro-inflammatory and/or tolerizing responses. Methods.– Colitis was induced in C57/B6 donor mice by intracolonic instillation of trinitrobenzenesulfonic acid (TNBS). Donor mice received five oral doses of colonic proteins extracted from TNBS-colitis colonic wall. Depletion of NK1.1+ lymphocytes was performed before lymphocyte harvesting. Splenocytes were harvested and separated into T-cell subpopulations, and transplanted into recipient mice before intracolonic instillation of TNBS. Standard clinical, macroscopic, and microscopic scores, and intracellular staining, flow cytometry, and cytotoxicity assays were performed. Results.– The adoptive transfer of CD4+ and NK1.1+ cells harvested from tolerized mice markedly ameliorated the colitis in recipient mice. In contrast, the adoptive transfer of CD8+ and double negative lymphocytes failed to transfer the tolerance. Recipients of splenocytes from tolerized mice exhibited an increase in CD4+IL4+/CD4+IFNγ+ ratio. In contrast, recipients of splenocytes from NK1.1-depleted-tolerized mice exhibited severe colitis with a significant decrease of the CD4+IL4+/CD4+IFNγ+ ratio. However adoptive transfer of splenocytes from non-tolerized NKT-depleted mice led to an alleviation of colitis with a relative increase of the CD4+IL4+/CD4+IFNγ+ ratio. Conclusions: NK1.1+ lymphocytes play a critical role in immune regulation. They may be accountable for an alteration of the inflammatory response and the CD4+IL4+/CD4+IFNγ ratio immune-mediated colitis and in peripheral tolerance induction. |
| Databáze: | OpenAIRE |
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