Eternal sunshine of the spotless cycle
| Autor: | Lendert Gelens, Silvia D.M. Santos |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
DNA Replication
Medicine (General) Biochemistry & Molecular Biology QH301-705.5 Cell cycle progression Biology General Biochemistry Genetics and Molecular Biology Cell cycle phase 03 medical and health sciences 0302 clinical medicine R5-920 Cyclin D Humans News & Views Biology (General) Quantitative Biology & Dynamical Systems 030304 developmental biology 0303 health sciences Science & Technology General Immunology and Microbiology Applied Mathematics Cell Cycle Cyclin-Dependent Kinase 2 Temperature Oncogenes Cell cycle Cell biology Computational Theory and Mathematics Cell cycle control General Agricultural and Biological Sciences Life Sciences & Biomedicine 030217 neurology & neurosurgery DNA Damage Information Systems |
| Zdroj: | Molecular Systems Biology, Vol 15, Iss 4, Pp n/a-n/a (2019) Molecular Systems Biology |
| Popis: | The cell cycle is canonically described as a series of four consecutive phases: G1, S, G2, and M. In single cells, the duration of each phase varies, but the quantitative laws that govern phase durations are not well understood. Using time-lapse microscopy, we found that each phase duration follows an Erlang distribution and is statistically independent from other phases. We challenged this observation by perturbing phase durations through oncogene activation, inhibition of DNA synthesis, reduced temperature, and DNA damage. Despite large changes in durations in cell populations, phase durations remained uncoupled in individual cells. These results suggested that the independence of phase durations may arise from a large number of molecular factors that each exerts a minor influence on the rate of cell cycle progression. We tested this model by experimentally forcing phase coupling through inhibition of cyclin-dependent kinase 2 (CDK2) or overexpression of cyclin D. Our work provides an explanation for the historical observation that phase durations are both inherited and independent and suggests how cell cycle progression may be altered in disease states. |
| Databáze: | OpenAIRE |
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