IRF9-Stat2 Fusion Protein as an Innate Immune Inducer to Activate Mx and Interferon-Stimulated Gene Expression in Zebrafish Larvae
| Autor: | Yimin Wu, Chang Jen Huang, Huang Wei Lien, Chih Ming Chou, Cheng Ying Chu, Jhih Yun Ho, Chia Hsiung Cheng |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Myxovirus Resistance Proteins Transcriptional Activation animal structures Recombinant Fusion Proteins Applied Microbiology and Biotechnology 03 medical and health sciences Transactivation Transcription (biology) Chlorocebus aethiops Animals Electrophoretic mobility shift assay STAT2 Promoter Regions Genetic Zebrafish Innate immune system biology Interferon-stimulated gene fungi STAT2 Transcription Factor Interferon-Stimulated Gene Factor 3 biology.organism_classification Fusion protein Molecular biology Immunity Innate 030104 developmental biology Gene Expression Regulation Larva COS Cells biology.protein Signal Transduction |
| Zdroj: | Marine biotechnology (New York, N.Y.). 19(3) |
| ISSN: | 1436-2236 |
| Popis: | Virus infection often causes large amounts of mortality during teleost larvae stage. Strong induction of innate immunity to increase survival rates of teleost larvae has been less reported. In this study, we present a zebrafish IRF9-Stat2 fusion protein (zIRF9-S2C) as a strong innate immunity inducer and characterized induction of interferon-stimulated genes (ISGs) in zebrafish larvae. zIRF9-S2C could mimic IFN-stimulated gene factor 3 (ISGF3) complex to constitutively activate transcription of Mx promoter through IFN-stimulatory element (ISRE) sites. Mutation of two ISRE sites on Mx promoter reduced transactivation activities of Mx promoter induced by zIRF9-S2C. An electrophoretic mobility shift assay experiment shows that zIRF9-S2C could directly bind to two ISRE sites of Mx promoter. Induction of transactivation of Mx promoter by zIRF9-S2C shows significantly higher activity than by zebrafish IFN1 (zIFN1), IFNγ (zIFNγ), and Tetraodon IRF9-S2C (TnIRF9-S2C). zIRF9-S2C raises transcription of Mxa, Mxb, Mxc, Ifnφ1, Ifnφ2, and Ifnφ3 in zebrafish liver ((ZFL) cell line) cells and zebrafish larvae. Collectively, we suggest that IRF9-S2C could activate transcription of ISGs with species-specific recognition and could be an innate immunity inducer in teleost larvae. |
| Databáze: | OpenAIRE |
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