Identification of a novel food-derived collagen peptide, hydroxyprolyl-glycine, in human peripheral blood by pre-column derivatisation with phenyl isothiocyanate

Autor:	Eun Young Park, Kenji Sato, Saeko Akaba, Yasushi Nakamura, Eriko Kawashima, Yasutaka Shigemura
Rok vydání:	2011
Předmět:	chemistry.chemical_classification animal structures Chromatography integumentary system Collagen peptide Chemistry Phenyl isothiocyanate Hydroxyprolyl-glycine Peptide General Medicine Peripheral blood Hydrolysate Analytical Chemistry chemistry.chemical_compound Biochemistry Ingestion Food Science
Zdroj:	Food chemistry. 129(3)
ISSN:	0308-8146
Popis:	Peptides in the blood of subjects before and after collagen hydrolysate ingestion were fractionated by ion exchange and size-exclusion chromatographies and then derivatised with phenyl isothiocyanate. The derivatives were characterised by reserved phase (RP)-HPLC. Prolyl-hydroxyproline (Pro-Hyp), which has been identified in the previous studies, was detected as a major food-derived collagen peptide in the blood of all subjects (n=5). Another major peptide was identified as hydroxyprolyl-glycine (Hyp-Gly) in the blood of four subjects, which has not been detected in previous studies. The ratio of Hyp-Gly to Pro-Hyp depended on subjects and ranged from 0.00 to 5.04. Hyp-Gly was less susceptible to human serum peptidase than Pro-Hyp. Hyp-Gly enhanced the growth of mouse primary fibroblasts on collagen gels in a higher extent than Pro-Hyp. These findings suggest that Hyp-Gly plays a significant role in exerting the biological effects by ingestion of collagen hydrolysate.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7568d8d7bad0c4da415d6ca91fe89c9b https://pubmed.ncbi.nlm.nih.gov/25212331 Zobrazit plný text záznamu Full Text from ScienceDirect