In vitro neutralization of T-2 toxin toxicity by a monoclonal antibody
| Autor: | Fiorenza Minervini, Lldiko Bama‐Vetro, Giacomo Lucivero, Angelo Visconti, Lazlo Solti, Agnes Gyongyosi‐Horvath |
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| Přispěvatelé: | Minervini, F, GYONGYOSI HORVATH, A, Lucivero, Giacomo, Visconti, A, BARNA VETRO, I, Solti, L. |
| Jazyk: | angličtina |
| Rok vydání: | 1994 |
| Předmět: |
medicine.drug_class
Enzyme-Linked Immunosorbent Assay Toxicology medicine.disease_cause Monoclonal antibody Neutralization Mice Antibody Specificity Neutralization Tests medicine Animals Humans Lymphocytes Cytotoxicity Cells Cultured Toxin Chemistry Mtt method Antibodies Monoclonal Molecular biology In vitro T-2 Toxin Immunoglobulin G Monoclonal Toxicity Female Trichothecenes |
| Popis: | A T-2 toxin specific monoclonal antibody, IgGl K, with a low level of ELISA cross-reactivity to Acetyl T-2, HT-2, and iso T-2 toxins has been produced. The ability of this monoclonal antibocly to neutralize the cytotoxicity of T-2 toxin in PHA stimulated cultures of human lymphocytes was determined by the MTT method. The complete neutralization of the toxic effect of 0.02 μM T-2 toxin was obtained with 0.03 μM of MoAb, whereas the 50% neutralizing dose (ND50) was observed at 0.009 of MoAb. Partial neutralization was observed with Acetyl T-2 toxin (ND, = 0.038 μM) and HT-2 (ND50 = 0.94 μM). These results could represent a rational for clinical use of T-2 toxin specific monoclonal antibody in prophylaxis and therapy of T-2 toxemia. © 1994 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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