Complement component C3a or C3a desArg as a new marker for estimation of local vital reactions in incised skin wounds
| Autor: | Fukutaro Takabe, Hiroyuki Inoue, Yuka Mori, Mineo Iwasa, Yoshitaka Maeno |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Male
Pathology medicine.medical_specialty integumentary system Skin wound business.industry Guinea Pigs Immunoblotting chemical and pharmacologic phenomena C3a desarg Pathology and Forensic Medicine Complement system Molecular Weight Postmortem Changes Complement C3a Medicine Animals Anaphylatoxin Electrophoresis Polyacrylamide Gel Wound edge business Law Biomarkers Skin |
| Zdroj: | Forensic science international. 55(1) |
| ISSN: | 0379-0738 |
| Popis: | The anaphylatoxin C3a or its desArg form ( C3a desArg ) generated during complement activation could be detected in the vicinity of incised skin wounds of guinea pigs using immunoblotting methods. The C3a desArg peptides were detectable immediately after injury in local sites up to 3 mm from the wound edge. In subsequent determinations of up to at least 3-day-old antemortem wounds, the maximum concentration of these peptides was largely localized up to 6 mm from the wound edge at 2 h after injury. In postmortem wounds, however, these peptides were undetectable. When they were released in antemortem wounded tissues they could be detected up to 1 day at 22°C after death. These results suggest that the detection of C3a desArg in wounds using immunoblotting methods can be useful for distinguishing ante- from postmortem wounds. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|