Differential Expression of Extracellular Matrix and Adhesion Molecules in Fetal-Origin Amniotic Epithelial Cells of Preeclamptic Pregnancy

Autor: Min Young Lee, Ji Hea Yu, Sung Rae Cho, Young Han Kim, Mingi Kim, Ah Leum Kim, Mi Hyun Jo, Myung Sun Kim
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Embryology
Molecular biology
Maternal Health
Placenta
Gene Expression
lcsh:Medicine
Biochemistry
0302 clinical medicine
Sequencing techniques
Pre-Eclampsia
Pregnancy
Medicine and Health Sciences
Gene Regulatory Networks
lcsh:Science
Cells
Cultured
Multidisciplinary
Cell adhesion molecule
Obstetrics and Gynecology
RNA sequencing
Genomics
Complementary DNA
Extracellular Matrix
Nucleic acids
medicine.anatomical_structure
030220 oncology & carcinogenesis
Amniotic epithelial cells
Female
Anatomy
Transcriptome Analysis
Signal Transduction
Research Article
Adult
Adhesion Molecules
Biological adhesion
Forms of DNA
Biology
Preeclampsia
Andrology
03 medical and health sciences
medicine
Genetics
Humans
Amnion
Fetus
Sequence Analysis
RNA
Gene Expression Profiling
lcsh:R
Reproductive System
Trophoblast
Biology and Life Sciences
Computational Biology
Epithelial Cells
DNA
Molecular Development
medicine.disease
Genome Analysis
Pregnancy Complications
Research and analysis methods
030104 developmental biology
Molecular biology techniques
Gene Expression Regulation
Immunology
Women's Health
lcsh:Q
Cell Adhesion Molecules
Developmental Biology
Zdroj: PLoS ONE, Vol 11, Iss 5, p e0156038 (2016)
PLOS ONE(11): 5
PLoS ONE
ISSN: 1932-6203
Popis: Preeclampsia is a common disease that can occur during human pregnancy and is a leading cause of both maternal and neonatal morbidity and mortality. Inadequate trophoblast invasion and deficient remodeling of uterine spiral arteries are associated with preeclampsia (PE). The development of this syndrome is thought to be related to multiple factors. Recently, we isolated patient-specific human amniotic epithelial cells (AECs) from the placentas of 3 women with normal pregnancy and 3 with preeclamptic pregnancy. Since the characteristics of human AECs in PE are different from those in normal pregnancy, we sought to confirm the genes differentially expressed between preeclamptic pregnancy and normal pregnancy. Therefore, we performed transcriptome analysis to investigate the candidate genes associated with the possible pathophysiology of preeclampsia. Pathway analysis was performed using the Database for Annotation, Visualization, and Integrated Discovery (DAVID) and Kyoto Encyclopedia of Genes and Genomes (KEGG) online resource. In this study, we selected a total of 12 pathways and focused on extracellular matrix-related and biological adhesion molecules. Using RT-PCR array and real-time PCR, we confirmed that COL16A1, ITGB2, and LAMA3 were significantly up-regulated, but ITGA1, ITGA3, ITGA6, MMP1, MMP3, MMP10 and MMP11 were significantly down-regulated in preeclamptic fetal origin cells. Taken together, we suggest that the genes and pathways identified here may be responsible for the occurrence and development of PE, and controlling their expression may play a role in communication with fetal-maternal placenta to keep normal pregnancy.
Databáze: OpenAIRE
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