Characterization of Anti-Dal Alloantibodies Following Sensitization of Two Dal-Negative Dogs
| Autor: | S Goulet, M C Blais |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
040301 veterinary sciences
030204 cardiovascular system & hematology 0403 veterinary science 03 medical and health sciences Dogs 0302 clinical medicine Isoantibodies Animals Medicine Blood Transfusion Sensitization Blood type General Veterinary biology business.industry Immunogenicity 04 agricultural and veterinary sciences Agglutination (biology) Titer medicine.anatomical_structure Blood Grouping and Crossmatching Polyclonal antibodies Immunology biology.protein Female Immunization Antibody business Packed red blood cells |
| Zdroj: | Veterinary Pathology. 55:108-115 |
| ISSN: | 1544-2217 0300-9858 |
| DOI: | 10.1177/0300985816688746 |
| Popis: | Since its discovery, the immunogenicity of the Dal blood type has not been further investigated. The aim of this study was to better characterize anti- Dal alloantibodies produced following sensitization of Dal-negative dogs, notably their rate of appearance, the agglutination titer over time, and their immunoglobulin class. A secondary objective was to obtain polyclonal anti- Dal alloantibodies to increase the availability of Dal blood typing. Of 100 healthy laboratory Beagles tested, 2 Dal-negative dogs were identified as recipients. Ten healthy Dal-positive dogs were investigated as potential blood donors. All dogs were extensively blood typed for DEA 1, 3, 4, 5, and 7, as well as for Dal. Then, the recipients were transfused uneventfully with 10 ml/kg of Dal-positive but otherwise compatible packed red blood cells. Posttransfusion blood samples were collected routinely over a minimum of 1 year. Using a gel column technology, anti- Dal alloantibodies were detected as early as 4 days posttransfusion and remained detectable 2 years posttransfusion, with maximum agglutination titers reached at 1 and 2 months posttransfusion. The immunoglobulin class was IgG. The immunogenicity and clinical significance of the Dal blood type were confirmed. The results support the recommendations that previously transfused dogs be crossmatched starting 4 days posttransfusion and for the animal’s lifetime. The polyclonal anti- Dal antibodies produced will allow blood typing of a significant number of dogs, especially transfused dogs facing blood incompatibilities and canine blood donors. |
| Databáze: | OpenAIRE |
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