Cell Labeling via Membrane-Anchored Lipophilic MR Contrast Agents
| Autor: | David R. Kasjanski, Daniel J. Mastarone, Andy H. Hung, Keith W. MacRenaris, Christiane E. Carney, Thomas J. Meade |
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| Rok vydání: | 2014 |
| Předmět: |
Models
Molecular Surface Properties Stereochemistry Biomedical Engineering Contrast Media Pharmaceutical Science Gadolinium Bioengineering Article Cell membrane HeLa Mice In vivo Cell Line Tumor Organometallic Compounds medicine Animals Humans Chelation Particle Size Cell Proliferation Pharmacology Molecular Structure biology Chemistry Cell growth Cell Membrane Organic Chemistry biology.organism_classification Magnetic Resonance Imaging Small molecule In vitro medicine.anatomical_structure Membrane NIH 3T3 Cells Biophysics Hydrophobic and Hydrophilic Interactions HeLa Cells Biotechnology |
| Zdroj: | Bioconjugate Chemistry |
| ISSN: | 1520-4812 1043-1802 |
| DOI: | 10.1021/bc500083t |
| Popis: | Cell tracking in vivo with MR imaging requires the development of contrast agents with increased sensitivity that effectively label and are retained by cells. Most clinically approved Gd(III)-based contrast agents require high incubation concentrations and prolonged incubation times for cellular internalization. Strategies to increase contrast agent permeability have included conjugating Gd(III) complexes to cell penetrating peptides, nanoparticles, and small molecules which have greatly improved cell labeling but have not resulted in improved cellular retention. To overcome these challenges, we have synthesized a series of lipophilic Gd(III)-based MR contrast agents that label cell membranes in vitro. Two of the agents were synthesized with a multiplexing strategy to contain three Gd(III) chelates (1 and 2) while the third contains a single Gd(III) chelate (3). These new agents exhibit significantly enhanced labeling and retention in HeLa and MDA-MB-231-mcherry cells compared to agents that are internalized by cells (4 and Prohance). |
| Databáze: | OpenAIRE |
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