Assessment and characterization of the bacterial community structure in advanced activated sludge systems
| Autor: | Daniele Di Trapani, Walter Arancio, Renato Fani, Giovanni Bacci, Paolo Cinà, Giorgio Mannina, Anna Maria Puglia, Giuseppe Gallo |
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| Přispěvatelé: | Cina P., Bacci G., Arancio W., Gallo G., Fani R., Puglia A.M., Di Trapani D., Mannina G. |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Environmental Engineering IFAS-MBR Biomass Bioengineering Wastewater treatment 010501 environmental sciences Membrane bioreactor 01 natural sciences Bioreactors 010608 biotechnology Rhodobacteraceae DNA extraction Waste Management and Disposal NGS of 16S rDNA amplicon 0105 earth and related environmental sciences Sewage Settore ICAR/03 - Ingegneria Sanitaria-Ambientale biology Renewable Energy Sustainability and the Environment Chemistry Microbiota Biofilm General Medicine biology.organism_classification Pulp and paper industry Anoxic waters Activated sludge Biofilms Sewage treatment 16S rRNA gene Bacterial community Bacteria |
| Zdroj: | Bioresource Technology. 282:254-261 |
| ISSN: | 0960-8524 |
| DOI: | 10.1016/j.biortech.2019.03.018 |
| Popis: | The present study is aimed to assess and characterize the structure of bacterial community in advanced activated sludge systems. In particular, activated sludge samples were collected from an Integrated Fixed-film Activated Sludge – Membrane Bioreactor pilot plant under a University of Cape Town configuration with in-series anaerobic (Noair)/anoxic (Anox)/aerobic (Oxy) reactors – and further analyzed. The achieved results – based on Next Generation Sequencing (NGS) of 16S rDNA amplicons – revealed that the bacterial biofilm (bf) communities on plastic carriers of Oxy and Anox reactors had a greater diversity compared to suspended (sp) bacterial flocs of Oxy, Anox and Noair. Indeed, the Shannon diversity indices of both biofilm communities were higher than those of suspended growth samples (Oxy-bf = 4.1 and Anox-bf = 4.2 vs. Oxy-sp = 3.4, Anox-sp = 3.5 and Noair-sp = 3.4). The most striking differences have been reported in Rhodobacteraceae being more abundant in biofilm specimens than in suspended biomass samples. The vast majority of the identified bacteria differs from those obtained by culture dependent method, thus suggesting that NGS-based method is really suitable to analyze the bacterial community composition, even in advanced systems for wastewater treatment. |
| Databáze: | OpenAIRE |
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