A potential role of mesenchymal stem cells derived from human umbilical cord blood in ameliorating psoriasis-like skin lesion in the rats
| Autor: | M A Fetouh, H F Abdel Hamid, M A Amin, M Rafla, Nagwa El-Nefiawy, S S Attia |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Pathology medicine.medical_specialty Histology medicine.medical_treatment H&E stain Betamethasone Dermis Psoriasis medicine Animals Basement membrane business.industry Mesenchymal stem cell Mesenchymal Stem Cells Stem-cell therapy medicine.disease Fetal Blood Rats medicine.anatomical_structure Epidermis Anatomy business medicine.drug |
| Zdroj: | Folia morphologica. 81(3) |
| ISSN: | 1644-3284 |
| Popis: | Background: Psoriasis is a common autoimmune inflammatory skin disease, with no clear cause, treated with topical agents and phototherapy to conventional immunosuppressant drugs and biologic agents. Stem cell therapy has showed significant interest in regenerative medicine. The aim of this study was to use mesenchymal stem cell (MSC) therapy compared to the topical application of the standard conventional corticosteroid cream. Materials and methods: 40 male adult albino rats were used, divided into 4 groups, 10 rats each. Group I (control), Group II (Psoriasis-like lesions induced by usage of Aldara cream), Group III (treated with Betamethasone) and Group IV (treated with mesenchymal stem cells). Specimens were stained with Hematoxylin & Eosin, Masson's trichrome, immune-histochemical technique for CD4, CD8 and CD31. Ultra-sections were prepared for transmission electron microscope examination. Results: MSCs demonstrated efficacy in reduction of disease severity in the form of uniform epidermal thickness covered by a very thin keratin layer. Normally- arranged layers of epidermal layers, with a clear border demarcation was seen between the epidermis and the dermis with apparently intact basement membrane. TEM showed absence of gaps between the tightly connected cells of the basal layer and the resting basement membrane. Conclusions: application of MSCs raises hope for developing a new, safe and effective therapy for psoriatic patients, avoiding the side effects of betamethasone. |
| Databáze: | OpenAIRE |
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