Classical Molecular Tests Using Urine Samples as a Potential Screening Tool for Human Papillomavirus Detection in Human Immunodeficiency Virus-Infected Women
| Autor: | Antonio Perez-Prados, Manuel E. Patarroyo, Sara C. Soto-De Leon, Andrea C. Pineda-Peña, Marina Muñoz, Manuel A. Patarroyo, Ricardo Sánchez, Milena Camargo |
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| Přispěvatelé: | Universidad Pública de Navarra. Departamento de Estadística e Investigación Operativa, Nafarroako Unibertsitate Publikoa. Estatistika eta Ikerketa Operatiboa Saila |
| Rok vydání: | 2013 |
| Předmět: |
Urine samples
medicine.medical_treatment Prevalence HIV Infections Cervix Uteri Urine Polymerase Chain Reaction law.invention Human papillomavirus type 18 Human papillomavirus type 6 PCR-based moleculat tests Human papillomavirus type 58 law Human papillomavirus type 16 Mixed infection Viral Human papillomavirus type 11 Papillomaviridae Polymerase chain reaction Priority journal Virus detection Cervical cancer HPV infection Immunosuppression Middle Aged Molecular Diagnostic Techniques Female Human Adult Microbiology (medical) Major clinical study Papillomavirus infection Urinalysis Uterine cervix cytology Biology Sensitivity and Specificity Article Cancer screening Wart virus Human immunodeficiency virus infection Virology Virus DNA medicine Humans Human papillomavirus type 31 Human papillomavirus type 33 DNA Primers Human papillomavirus (HPV) Papillomavirus Infections HIV DNA Nonhuman medicine.disease biology.organism_classification DNA Viral Immunology Human papillomavirus type 45 Controlled study Ascus |
| Zdroj: | Academica-e: Repositorio Institucional de la Universidad Pública de Navarra Universidad Pública de Navarra Repositorio EdocUR-U. Rosario Universidad del Rosario instacron:Universidad del Rosario Academica-e. Repositorio Institucional de la Universidad Pública de Navarra instname |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.01302-13 |
| Popis: | Incluye un fichero de datos Human papillomavirus (HPV) is the main risk factor associated with the development of cervical cancer (CC); however, there are other factors, such as immunosuppression caused by the human immunodeficiency virus (HIV), that favor progression of the illness. This study was thus aimed at evaluating the functionality of classical PCR-based molecular tests for the generic identification of HPV DNA (GP5 /GP6 , MY09/MY11, and pU1M/2R primers, individually or in combination) using cervical and urine samples from 194 HIV-positive women. Infected samples were tested with type-specific primers for six high-risk types (HPV-16, -18, -31, -33, -45, and -58) and two low-risk types (HPV-6 and -11). HPV infection prevalence rates were 70.1% for the cervical samples and 63.9% for the urine samples. HPV-16 was the most prevalent viral type in the cervical and urine samples, with higher rates of multiple infections than single infections detected in such samples. HPV DNA detection by PCR (mainly with the pU1M/2R primer set) in urine samples was positively associated with abnormal cytological findings (atypical squamous cells of undetermined significance/squamous intraepithelial lesions [ASCUS/SIL]). It was determined that the operative characteristics for detection of cytological abnormalities were similar for cervical and urine samples. This suggested using PCR for the detection of HPV DNA in urine samples as a potential screening strategy for CC prevention in future prevention and control programs along with currently implemented strategies for reducing the impact of the disease, i.e., urine samples are economical, are easy to collect, have wide acceptability among women, and have operative characteristics similar to those of cervical samples. The authors are grateful to the Basque Cooperation Agency for Development and the Spanish Agency for International Development Cooperation (AECID) for supporting and financing this project. |
| Databáze: | OpenAIRE |
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