Molecular cloning, expression, and regulation of estrogen receptors in pigeon oviduct epithelial cells
| Autor: | Z.R. Tao, Jian Li, Huilai Zhang, G.L. Li, Y.Y. Ding, S.L. Zhong, Fang Chen, L.Z. Lu |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Gene isoform
medicine.medical_specialty Cell type medicine.drug_class Gene Expression Estrogen receptor Ovary Oviducts Biology Internal medicine Genetics medicine Animals Estrogen Receptor beta RNA Messenger Cloning Molecular Columbidae Molecular Biology Estrogen receptor beta Estradiol Estrogen Receptor alpha food and beverages Epithelial Cells General Medicine Cell biology Endocrinology medicine.anatomical_structure Estrogen Oviduct Female Estrogen receptor alpha |
| Zdroj: | Genetics and Molecular Research. 13:1926-1937 |
| ISSN: | 1676-5680 |
| Popis: | Estrogen regulates reproductive behavior and drives the proliferation and differentiation of several cell types. These physiological functions of estrogen are mediated by estrogen receptors (ERs), and each ER isoform plays a distinct role. To clarify the molecular mechanism of estrogen action and to evaluate the effect of ERs on the secretion of ovalbumin (OVA) in pigeon oviduct epithelial cells (POECs), we determined the complete coding sequences encoding ER alpha (ERα) and ER beta (ERβ) in pigeons. The abundance of pigeon ERα and ERβ mRNA was detected using quantitative polymerase chain reaction. These results revealed that pigeon ERα is highly expressed in the oviduct, while pigeon ERb is highly expressed in the ovary and kidney. We hypothesize that ERα mRNA predominates over that of ERβ in the oviduct. The expression of ERα can be down-regulated by 17β-estradiol, and the knockdown of ERα promoted OVA mRNA expression in cultured POECs, indicating that ERα may play an important role in OVA secretion. |
| Databáze: | OpenAIRE |
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