| Autor: |
Spiteri, Alanna G., Terry, Rachel L., Wishart, Claire L., Ashhurst, Thomas M., Campbell, Iain L., Hofer, Markus J., King, Nicholas J. C. |
| Rok vydání: |
2021 |
| Předmět: |
|
| DOI: |
10.6084/m9.figshare.15058126 |
| Popis: |
Additional file 9 ‘Microglia-specific’ marker P2RY12 is expressed by infiltrating macrophages populations in the brain but not in the bone marrow or blood. a, b Histograms showing the expression of P2RY12 on microglia (a) in naïve and WNV-infected mice and on microglia, Ly6Chi macrophages and microglia-like macrophages (b) in WNV dpi 7 brains. Fluorescence minus one (FMO) and single stains (ss) for P2RY12 are shown each plot. c-f tSNE plot clustered on live cells from naïve and WNV dpi 7 brains. c, d Annotation of populations found in the mock-infected (c) and infected (d) brain tSNE. e, f Relative expression P2RY12 on tSNE plots representing naïve (e) and WNV-dpi 7 (f) brains. g Heatmap showing the expression P2RY12 on neutrophils, microglia, microglia-like macrophages and Ly6Chi macrophages from four brain samples (1-4). h Expression of P2RY12 on peripheral bone marrow and blood Ly6Chi monocytes and Ly6Chi macrophages in WNV dpi 7 brains. i-k tSNE plot clustered on live, SSC-Alo and Ly6G+/- bone marrow, live, SSC-Alo and Ly6G+/- blood cells and live brain cells from WNV dpi 7 brains. Data is representative of at least two independent experiments, with a minimum 7 animals per group. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
