Role of the area postrema in the hypophagic effects of oleoylethanolamide

Autor: Cristina Anna Gallelli, Carlo Cifani, Justyna Barbara Koczwara, Silvana Gaetani, Adele Romano, Thomas A. Lutz, Maria Vittoria Micioni Di Bonaventura, Mario Falchi, Tommaso Cassano, Fiona E. Braegger, Annabella Vitalone
Přispěvatelé: University of Zurich, Gaetani, Silvana
Rok vydání: 2017
Předmět:
Male
0301 basic medicine
medicine.medical_specialty
satiety
Hypothalamus
feeding behavior
Oleic Acids
Dopamine beta-Hydroxylase
Oxytocin
oleoylethanolamide: (PubChem CID: 5283454)
area postrema
brainstem
hypothalamus
nucleus of the solitary tract
Eating
03 medical and health sciences
Oleoylethanolamide
chemistry.chemical_compound
0302 clinical medicine
Neurochemical
Internal medicine
medicine
Animals
PPAR alpha
Rats
Wistar
Pharmacology
Area postrema
Solitary tract
10081 Institute of Veterinary Physiology
Endocannabinoid system
3004 Pharmacology
030104 developmental biology
Endocrinology
Area Postrema
chemistry
10076 Center for Integrative Human Physiology
570 Life sciences
biology
Brainstem
Proto-Oncogene Proteins c-fos
030217 neurology & neurosurgery
Brain Stem
Endocannabinoids
medicine.drug
Zdroj: Pharmacological Research. 122:20-34
ISSN: 1043-6618
DOI: 10.1016/j.phrs.2017.05.017
Popis: The satiety-promoting action of oleoylethanolamide (OEA) has been associated to the indirect activation of selected brain areas, such as the nucleus of the solitary tract (NST) in the brainstem and the tuberomammillary (TMN) and paraventricular (PVN) nuclei in the hypothalamus, where noradrenergic, histaminergic and oxytocinergic neurons play a necessary role. Visceral ascending fibers were hypothesized to mediate such effects. However, our previous findings demonstrated that the hypophagic action of peripherally administered OEA does not require intact vagal afferents and is associated to a strong activation of the area postrema (AP). Therefore, we hypothesized that OEA may exert its central effects through the direct activation of this circumventricular organ. To test this hypothesis, we subjected rats to the surgical ablation of the AP (APX rats) and evaluated the effects of OEA (10mgkg-1 i.p.) on food intake, Fos expression, hypothalamic oxytocin (OXY) immunoreactivity and on the expression of dopamine beta hydroxylase (DBH) in the brainstem and hypothalamus. We found that the AP lesion completely prevented OEA's behavioral and neurochemical effects in the brainstem and the hypothalamus. Moreover OEA increased DBH expression in AP and NST neurons of SHAM rats while the effect in the NST was absent in APX rats, thus suggesting the possible involvement of noradrenergic AP neurons. These results support the hypothesis of a necessary role of the AP in mediating OEA's central effects that sustain its pro-satiety action.
Databáze: OpenAIRE
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