Evidence for hybrid NMDA/kainate receptors from protein reconstitution studies and expression of vertebrate CNS RNAs in Xenopus oocytes
| Autor: | P.N.R. Usherwood, E.A. Barnard |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
medicine.medical_specialty
N-Methylaspartate Immunology Protein reconstitution Xenopus Glycine Kainate receptor AMPA receptor Biology Receptors N-Methyl-D-Aspartate Chromatography Affinity Xenopus laevis Receptors Kainic Acid Internal medicine medicine Animals RNA Messenger Long-term depression Pharmacology Kainic Acid Glutamate receptor biology.organism_classification Cell biology Endocrinology Receptors Glutamate Oocytes NMDA receptor Ion Channel Gating |
| Zdroj: | Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology. 103(1) |
| ISSN: | 0742-8413 |
| Popis: | 1. A review is presented of recent advances in glutamate receptor research with particular emphasis on studies which show that some glutamate receptors in the central nervous systems (CNS) of Xenopus and rat contain a mixture of N- methyl - D - aspartate-sensitive and kainate-sensitive subunits. 2. Protein isolated from Xenopus CNS using a domoic acid affinity column exhibits complex pharmacological properties. It binds both [3H]kainate and [3H]glycine: the binding of the latter is strychnine-insensitive. 3. When reconstituted into lipid bilayers, channels gated by kainate and NMDA can be elicited and the properties of these channels are similar to those gated by kainate receptors and NMDA receptors, respectively, in studies of vertebrate central neurones in situ. 4. The protein can be fractionated into two components; one of which is sensitive only to kainate and AMPA, the other exhibiting sensitivity to both kainate and NMDA. 5. When RNA isolated from Xenopus and rat CNS is injected into Xenopus oocytes, responses to kainate and NMDA can be seen within 2–3 days. The responses to co-application of these agonists support the contention that some of the glutamate receptors expressed in oocytes contain both kainate-sensitive and NMDA-sensitive subunits. |
| Databáze: | OpenAIRE |
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