Junctional adhesion molecule 2 mediates the interaction between hatched blastocyst and luminal epithelium: induction by progesterone and LIF
| Autor: | Shun-Li Yue, Xuhui Feng, Ren-Wei Su, Hua Ni, Zeng-Ming Yang, Weng-Bo Deng, Bo Jia, Zhen-Ao Zhao, Tong-Song Wang, Wei Lei, Ji-Long Liu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Anatomy and Physiology
lcsh:Medicine Cell junction Leukemia Inhibitory Factor Biochemistry Epithelium Mice Pregnancy Reproductive Physiology Molecular Cell Biology STAT3 lcsh:Science Progesterone Multidisciplinary biology Cell adhesion molecule Adhesion Mifepristone Cell biology Extracellular Matrix medicine.anatomical_structure Intercellular Junctions Cytochemistry Medicine Female medicine.drug Research Article medicine.medical_specialty Endocrine System Internal medicine medicine Cell Adhesion Animals Reproductive Endocrinology Blastocyst Embryo Implantation Biology Extracellular Matrix Adhesions Endocrine Physiology urogenital system Uterus lcsh:R Reproductive System Endocrinology biology.protein lcsh:Q Leukemia inhibitory factor Cell Adhesion Molecules |
| Zdroj: | PLoS ONE, Vol 7, Iss 4, p e34325 (2012) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | BACKGROUND: Junctional adhesion molecule 2 (Jam2) is a member of the JAM superfamily. JAMs are localized at intercellular contacts and participated in the assembly and maintenance of junctions, and control of cell permeability. Because Jam2 is highly expressed in the luminal epithelium on day 4 of pregnancy, this study was to determine whether Jam2 plays a role in uterine receptivity and blastocyst attachment in mouse uterus. METHODOLOGY/PRINCIPAL FINDINGS: Jam2 is highly expressed in the uterine luminal epithelium on days 3 and 4 of pregnancy. Progesterone induces Jam2 expression in ovariectomized mice, which is blocked by progesterone antagonist RU486. Jam2 expression on day 4 of pregnancy is also inhibited by RU486 treatment. Leukemia inhibitory factor (LIF) up-regulates Jam2 protein in isolated luminal epithelium from day 4 uterus, which is blocked by S3I-201, a cell-permeable inhibitor for Stat3 phosphorylation. Under adhesion assay, recombinant Jam2 protein increases the rate of blastocyst adhesion. Both soluble recombinant Jam2 and Jam3 can reverse this process. CONCLUSION: Jam2 is highly expressed in the luminal epithelium of receptive uterus and up-regulated by progesterone and LIF via tyrosine phosphorylation of Stat3. Jam2 may play a role in the interaction between hatched blastocyst and receptive uterus. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|