Detection and quantification of periodontal pathogens in smokers and never-smokers with chronic periodontitis by real-time polymerase chain reaction
| Autor: | Cláudio Mendes Pannuti, Giorgio De Micheli, Ecinele Francisca Rosa, Daniele Salami Lourenção, Mario Hiroyuki Hirata, E. F Gomes, Gislene Inoue, Mariana Rocha Guglielmetti, Fausto Medeiros Mendes, Rosario Dominguez Crespo Hirata |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Male medicine.medical_specialty Periodontal examination Bleeding on probing Dental Plaque Gingiva Dentistry Real-Time Polymerase Chain Reaction Gastroenterology Aggregatibacter actinomycetemcomitans Internal medicine Periodontal Attachment Loss medicine Tannerella forsythia Bacteroides Humans Periodontal Pocket Porphyromonas gingivalis Aged Carbon Monoxide biology Bacteria business.industry Smoking Treponema denticola Middle Aged biology.organism_classification medicine.disease Chronic periodontitis Bacterial Load Cross-Sectional Studies Clinical attachment loss Case-Control Studies Chronic Periodontitis Periodontics Female medicine.symptom business Gingival Hemorrhage |
| Zdroj: | Journal of periodontology. 85(10) |
| ISSN: | 1943-3670 |
| Popis: | The purpose of the present investigation is to compare the presence and number of periodontal pathogens in the subgingival microbiota of smokers versus never-smokers with chronic periodontitis and matched probing depths (PDs) using real-time polymerase chain reaction (RT-PCR).Forty current smokers and 40 never-smokers, matched for age, sex, and mean PD of sampling site, were included in this investigation. A full-mouth periodontal examination was performed, and a pooled subgingival plaque sample was collected from the deepest site in each quadrant of each participant. To confirm smoking status, expired carbon monoxide (CO) concentrations were measured with a CO monitor. The presence and quantification of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola were determined using RT-PCR.Smokers had greater overall mean PD (P = 0.001) and attachment loss (P = 0.006) and fewer bleeding on probing sites (P = 0.001). An association was observed between smoking status and the presence of A. actinomycetemcomitans (P0.001). The counts of A. actinomycetemcomitans (P0.001), P. gingivalis (P = 0.042), and T. forsythia (P0.001) were significantly higher in smokers.Smokers showed significantly greater amounts of P. gingivalis, A. actinomycetemcomitans, and T. forsythia than never-smokers. There was a significant association between smoking and the presence of A. actinomycetemcomitans. |
| Databáze: | OpenAIRE |
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