Pseudoalteromone A, a Ubiquinone Derivative from Marine Pseudoalteromonas spp., Suppresses Melanogenesis
| Autor: | Sang-Jip Nam, Heung-Soo Beak, So-Hee Kim, Eun-Soo Lee, Su-Jin Lim, Sung-Yoen Cho, Jaeyoung Ko, Daejin Min, Hyuk Kim, Chang Seok Lee, Jihye Lee |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
melanogenesis
Aquatic Organisms QH301-705.5 pseudoalteromone A Ubiquinone Tyrosinase Cell Pharmaceutical Science Antineoplastic Agents Article Melanin Pseudoalteromonas Western blot Pseudoalteromonas sp Cell Line Tumor Drug Discovery Extracellular medicine Animals Humans Biology (General) Pharmacology Toxicology and Pharmaceutics (miscellaneous) marine natural product biology medicine.diagnostic_test Chemistry Monophenol Monooxygenase biology.organism_classification Molecular biology medicine.anatomical_structure Cell culture Melanocytes Intracellular |
| Zdroj: | Marine Drugs Volume 19 Issue 11 Marine Drugs, Vol 19, Iss 612, p 612 (2021) |
| ISSN: | 1660-3397 |
| Popis: | An ubiquinone derivative, pseudoalteromone A (1), has been isolated from two marine-derived Pseudoalteromonas spp., APmarine002 and ROA-050, and its anti-melanogenesis activity was investigated. The anti-melanogenic capacity of pseudoalteromone A was demonstrated by assessing the intracellular and extracellular melanin content and cellular tyrosinase activity in the B16 cell line, Melan-a mouse melanocyte cell line, and MNT-1 human malignant melanoma cell line. Treatment with pseudoalteromone A (40 μg/mL) for 72 h reduced α-melanocyte-stimulating hormone (α-MSH)-induced intracellular melanin production by up to 44.68% in B16 cells and 38.24% in MNT-1 cells. Notably, pseudoalteromone A induced a concentration-dependent reduction in cellular tyrosinase activity in B16 cell, and Western blot analyses showed that this inhibitory activity was associated with a significant decrease in protein levels of tyrosinase and tyrosinase-related protein 1 (Tyrp-1), suggesting that pseudoalteromone A exerts its anti-melanogenesis activity through effects on melanogenic genes. We further evaluated the skin-whitening effect of pseudoalteromone A in the three-dimensional (3D) pigmented-epidermis model, MelanoDerm, and visualized the 3D distribution of melanin by two-photon excited fluorescence imaging in this human skin equivalent. Collectively, our findings suggest that pseudoalteromone A inhibits tyrosinase activity and expression and that this accounts for its anti-melanogenic effects in melanocytes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|