In vivo and in vitro effects of 1,1-dimethylhydrazine on selected immune functions
| Autor: | Richard G. Olsen, Melinda J. Tarr, Debra L. Jacobs |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Male
medicine.medical_specialty Lipopolysaccharide Lymphocyte Dose-Response Relationship Immunologic Hemolytic Plaque Technique Lymphocyte Activation T-Lymphocytes Regulatory law.invention Mice chemistry.chemical_compound Immune system In vivo law Internal medicine Dimethylhydrazine medicine Animals Cells Cultured Dimethylhydrazines Pharmacology Dose-Response Relationship Drug biology Molecular biology In vitro medicine.anatomical_structure Endocrinology chemistry Concanavalin A Antibody Formation biology.protein Suppressor Female Spleen Methylhydrazines |
| Zdroj: | Immunopharmacology. 4:139-147 |
| ISSN: | 0162-3109 |
| Popis: | The in vivo phase of the experiments reported here include the evaluation of immune function after short-or long-term treatment of mice with 1,1-dimethylhydrazine (UDMH). Long-term exposure (3 injections/week for 14 weeks) resulted in increased numbers of Jerne plaque-forming cells, a trend toward decreased induction of suppressor cell activity by concanavalin A (Con A), and no effects on mitogen-induced lymphocyte blast transformation (LBT), compared to saline-treated control mice. These effects were greatest at doses of 10 or 50 mg/kg, while higher doses had less of an effect. In vitro experiments were performed by adding UDMH to normal murine splenocytes in the LBT assay and con A-induced suppressor cell assay. The UDMH induced a significant enhanced response to lipopolysaccharide (LPS) at 10 and 50 micrograms/ml, and a suppressed response to both Con A and LPS at higher concentrations. The UDMH also caused a decrease in suppressor cell activity at 25 micrograms/ml. Selective abrogation of suppressor activity or alteration of the suppressor cell-helper ratio were suggested as possible mechanisms for the enhancement effect associated with UDMH. |
| Databáze: | OpenAIRE |
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