Development and validation of a UPLC–MS/MS method for the simultaneous determination of paritaprevir and ritonavir in rat liver

Autor: Andrew J. Ocque, Gene D. Morse, Robin DiFrancesco, Andrew H. Talal, Colleen E. Hagler, Yvonne Woolwine-Cunningham, Cindy J Bednasz
Rok vydání: 2016
Předmět:
Cyclopropanes
Male
On column
Macrocyclic Compounds
Proline
Lactams
Macrocyclic
Clinical Biochemistry
Pilot Projects
Hepacivirus
Tandem mass spectrometry
Antiviral Agents
01 natural sciences
High-performance liquid chromatography
Analytical Chemistry
Rats
Sprague-Dawley
03 medical and health sciences
0302 clinical medicine
Limit of Detection
Tandem Mass Spectrometry
immune system diseases
medicine
Animals
Protease Inhibitors
General Pharmacology
Toxicology and Pharmaceutics
Chromatography
High Pressure Liquid
Detection limit
Sulfonamides
Ritonavir
Chromatography
Chemistry
010401 analytical chemistry
Methodology
virus diseases
General Medicine
Hepatitis C
Rats
0104 chemical sciences
Medical Laboratory Technology
Liver
Paritaprevir
Rat liver
030211 gastroenterology & hepatology
Uplc ms ms
medicine.drug
Zdroj: Bioanalysis. 8:1353-1363
ISSN: 1757-6199
1757-6180
Popis: Aim: Determination of paritaprevir and ritonavir in rat liver tissue samples. Results: We successfully validated a UPLC–MS/MS method to measure paritaprevir and ritonavir in rat liver using deuterated internal standards (d8-paritapervir and d6-ritonavir). The method is linear from 20 to 20,000 and 5 to 10,000 pg on column for paritaprevir and ritonavir, respectively, and is normalized per milligram tissue. Interday and intraday variability ranged from 0.591 to 5.33% and accuracy ranged from -6.68 to 10.1% for quality control samples. The method was then applied to the measurement of paritaprevir and ritonavir in rat liver tissue samples from a pilot study. Conclusion: The validated method is suitable for measurement of paritaprevir and ritonavir within rat liver tissue samples for PK studies.
Databáze: OpenAIRE
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