Panel of suitable reference genes and its gender differences of fetal rat liver under physiological conditions and exposure to dexamethasone during pregnancy
Autor: | Liang Liu, Heze Liu, Hui Wang, Kexin Liu, Hui Han |
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Rok vydání: | 2021 |
Předmět: |
Male
Ribosomal Proteins Gestational Age 010501 environmental sciences Toxicology 01 natural sciences Dexamethasone Andrology 03 medical and health sciences stomatognathic system Pregnancy Reference genes RNA Ribosomal 18S medicine Animals Rats Wistar Sterol Regulatory Element Binding Protein 1c Glyceraldehyde 3-phosphate dehydrogenase 030304 developmental biology 0105 earth and related environmental sciences Sex Characteristics 0303 health sciences Fetus biology Gene Expression Regulation Developmental Glyceraldehyde-3-Phosphate Dehydrogenases medicine.disease Actins Rats Physiological model Liver Rat liver biology.protein Female Sterol Regulatory Element Binding Protein 1 medicine.drug |
Zdroj: | Reproductive Toxicology. 100:74-82 |
ISSN: | 0890-6238 |
Popis: | The panel of suitable reference genes in the fetal liver have not been reported. In this study, five commonly used reference genes (GAPDH, β-actin, Rn18 s, Rpl13a, and Rps29) were firstly selected as candidates. Bestkeeper, GeNorm, and NormFinder software were then used to screen out the panel of suitable reference genes of male and female fetal rat liver under physiological and prenatal dexamethasone exposure (PDE) conditions. Finally, we verified the reliability of the screened panel of reference genes by standardizing sterol regulatory element binding protein 1c (SREBP1c) expression with different reference genes. The results showed that GAPDH + Rn18 s and GAPDH + Rpl13a were respectively the panel of suitable reference genes in male and female rat fetal liver under the physiological model, while Rn18 s + Rps29 and GAPDH + Rn18 s were respectively under the PDE model. The results showed that different reference genes affected the statistical results of SREBP1c expression, and the screened panel of suitable reference genes under the PDE model had smaller intragroup differences, when compared with other reference genes under physiological and PDE models. In conclusion, we screened and determined that the panel of suitable reference genes were GAPDH + Rn18 s and Rn18 s + Rps29 in the male rat fetal liver under physiological and PDE models, while they were GAPDH + Rpl13a and GAPDH + Rn18 s in the females, and confirmed that the selection of the panel of suitable reference genes in the fetal liver had gender differences and pathological model specificity. |
Databáze: | OpenAIRE |
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