Identification of Hydrophobic Tags for the Degradation of Stabilized Proteins
| Autor: | Craig M. Crews, Thomas B. Sundberg, Taavi K. Neklesa, Devin J. Noblin, Kanak Raina, Hyun Seop Tae, Anke G. Roth, Jeffrey L. Gustafson |
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| Rok vydání: | 2012 |
| Předmět: |
Molecular Structure
Extramural Recombinant Fusion Proteins Organic Chemistry HEK 293 cells Chemical biology Biology Protein degradation Biochemistry Fusion protein Article Cell biology HEK293 Cells Proteostasis Biophysics Humans Molecular Medicine Degradation (geology) Molecule Hydrophobic and Hydrophilic Interactions Molecular Biology |
| Zdroj: | ChemBioChem. 13:538-541 |
| ISSN: | 1439-4227 |
| Popis: | New HyTs are a knockout: we previously reported that labeling HaloTag proteins with low molecular weight hydrophobic tags (HyTs) leads to targeted degradation of HaloTag fusion proteins. In this report, we employed a chemical approach to extend this hydrophobic tagging methodology to highly stabilized proteins by synthesizing and evaluating a library of HyTs, which led to the identification of HyT36. |
| Databáze: | OpenAIRE |
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