Two different specific JNK activators are required to trigger apoptosis or compensatory proliferation in response to Rbf1 in Drosophila
| Autor: | Aurore Rincheval-Arnold, Isabelle Guénal, Adrienne Baillet, Bernard Mignotte, Amandine Clavier |
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| Přispěvatelé: | Laboratoire de génétique et biologie cellulaire (LGBC), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Kinase [SDV]Life Sciences [q-bio] Mutant Signal transducing adaptor protein RAC1 Apoptosis Cell Biology JUN kinase [SDV.BC]Life Sciences [q-bio]/Cellular Biology Biology Phenotype Cell biology 03 medical and health sciences 030104 developmental biology dTRAF1 compensatory proliferation Report JNK Signal transduction Rbf1 Molecular Biology Rac1 Developmental Biology |
| Zdroj: | Cell Cycle Cell Cycle, Taylor & Francis, 2016, ⟨10.1080/15384101.2015.1100776⟩ |
| ISSN: | 1538-4101 1551-4005 |
| DOI: | 10.1080/15384101.2015.1100776⟩ |
| Popis: | The Jun Kinase (JNK) signaling pathway responds to diverse stimuli by appropriate and specific cellular responses such as apoptosis, differentiation or proliferation. The mechanisms that mediate this specificity remain largely unknown. The core of this signaling pathway, composed of a JNK protein and a JNK kinase (JNKK), can be activated by various putative JNKK kinases (JNKKK) which are themselves downstream of different adaptor proteins. A proposed hypothesis is that the JNK pathway specific response lies in the combination of a JNKKK and an adaptor protein upstream of the JNKK. We previously showed that the Drosophila homolog of pRb (Rbf1) and a mutant form of Rbf1 (Rbf1D253A) have JNK-dependent pro-apoptotic properties. Rbf1D253A is also able to induce a JNK-dependent abnormal proliferation. Here, we show that Rbf1-induced apoptosis triggers proliferation which depends on the JNK pathway activation. Taking advantage of these phenotypes, we investigated the JNK signaling involved in either Rbf1-induced apoptosis or in proliferation in response to Rbf1-induced apoptosis. We demonstrated that 2 different JNK pathways involving different adaptor proteins and kinases are involved in Rbf1-apoptosis (i.e. Rac1-dTak1-dMekk1-JNK pathway) and in proliferation in response to Rbf1-induced apoptosis (i.e., dTRAF1-Slipper-JNK pathway). Using a transient induction of rbf1, we show that Rbf1-induced apoptosis activates a compensatory proliferation mechanism which also depends on Slipper and dTRAF1. Thus, these 2 proteins seem to be key players of compensatory proliferation in Drosophila. |
| Databáze: | OpenAIRE |
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