Differences in steroid 5alpha-reductase iso-enzymes expression between normal and pathological human prostate tissue

Autor: Henriette Gérard, L'Houcine Ouafik, Jean-Pierre Raynaud, François Radvanyi, Pierre-Marie Martin, Sixtina Gil Diez de Medina, Dominique Chopin, Catherine Iehlé
Přispěvatelé: laboratoire de cancerologie experimentale INSERM CJF 93-11, INSERM, Ouafik, L'Houcine
Jazyk: angličtina
Rok vydání: 1999
Předmět:
Male
medicine.medical_specialty
Stromal cell
Endocrinology
Diabetes and Metabolism
Clinical Biochemistry
Prostatic Hyperplasia
[SDV.CAN]Life Sciences [q-bio]/Cancer
In situ hybridization
Biology
Biochemistry
Isozyme
MESH: Prostate
Epithelium
MESH: 3-Oxo-5-alpha-Steroid 4-Dehydrogenase
MESH: Prostatic Hyperplasia
Endocrinology
MESH: In Situ Hybridization
[SDV.CAN] Life Sciences [q-bio]/Cancer
3-Oxo-5-alpha-Steroid 4-Dehydrogenase
Prostate
Internal medicine
MESH: Reverse Transcriptase Polymerase Chain Reaction
Gene expression
medicine
Humans
RNA
Messenger
Molecular Biology
In Situ Hybridization
MESH: RNA
Messenger
Messenger RNA
MESH: Humans
Reverse Transcriptase Polymerase Chain Reaction
Prostatic Neoplasms
Cell Biology
Molecular biology
MESH: Male
Isoenzymes
MESH: Epithelium
medicine.anatomical_structure
Real-time polymerase chain reaction
MESH: Prostatic Neoplasms
MESH: Isoenzymes
Molecular Medicine
Zdroj: Journal of Steroid Biochemistry and Molecular Biology
Journal of Steroid Biochemistry and Molecular Biology, Elsevier, 1999, pp.189-95
ISSN: 0960-0760
Popis: International audience; We studied the expression level and cell-specific expression patterns of 5alpha-reductase (5alpha-R) types 1 and 2 iso-enzymes in human hyperplastic and malignant prostate tissue by semi-quantitative RT-PCR and in situ hybridisation analyses. In situ hybridisation established that 5alpha-R1 mRNA is preferentially expressed by epithelial cells and little expressed by stromal cells whereas 5alpha-R2 mRNA is expressed by both epithelium and stroma. Semi-quantitative RT-PCR has been performed on total RNA from different zones of normal prostate, BPH tissues and liver. We found that 5alpha-R1 and 5alpha-R2 mRNAs expression was near the same in all zones of normal prostate. In BPH tissue, 5alpha-R1 and 5alpha-R2 mRNAs expression was slightly but significantly increased, when it was compared to the levels recorded for normal prostate. In cancer samples, 5alpha-R1 mRNA expression was higher than in normal and hyperplastic prostate but the level of 5alpha-R2 mRNA was not statistically different from that observed in the different zones of normal prostate. In liver, 5alpha-R2 mRNA level was similar to that measured in BPH but 5alpha-R1 mRNA expression was ten times higher. The increase observed in 5alpha-R isoenzymes expression in BPH tissue could play an important role in the pathogenesis and/or maintenance of the disease.
Databáze: OpenAIRE
Externí odkaz: