Distinct behavioral and brain changes after different durations of the modified multiple platform method on rats: An animal model of central fatigue

Autor: Guo Siyuan, Yingqiu Mao, Chenxia Han, Yuehan Song, Jing Liu, Yan Liu, Jie Ma, Feng Li, Weihong Li
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Male
Physiology
Emotions
Social Sciences
lcsh:Medicine
Hippocampal formation
Anxiety
Pathology and Laboratory Medicine
Hippocampus
Nervous System
Biochemistry
Open field
chemistry.chemical_compound
0302 clinical medicine
Corticosterone
Medicine and Health Sciences
Hippocampus (mythology)
Psychology
lcsh:Science
Fatigue
Energy-Producing Organelles
Mammals
Multidisciplinary
Behavior
Animal
Brain
Animal Models
Mitochondria
Electrophysiology
Experimental Organism Systems
Neurology
Vertebrates
medicine.symptom
Anatomy
Cellular Structures and Organelles
Research Article
Elevated plus maze
medicine.medical_specialty
Neurophysiology
Biology
Bioenergetics
Research and Analysis Methods
Receptors
N-Methyl-D-Aspartate
Rodents
03 medical and health sciences
Animal model
Model Organisms
Signs and Symptoms
Diagnostic Medicine
Internal medicine
medicine
Animals
RNA
Messenger
Rats
Wistar
Maze Learning
CA1 Region
Hippocampal
Biological Locomotion
lcsh:R
Organisms
Biology and Life Sciences
Cell Biology
Rats
Sleep deprivation
Disease Models
Animal
030104 developmental biology
Endocrinology
chemistry
Gene Expression Regulation
Synapses
Amniotes
Sleep Deprivation
lcsh:Q
030217 neurology & neurosurgery
Multiple platform
Neuroscience
Zdroj: PLoS ONE, Vol 12, Iss 5, p e0176850 (2017)
PLoS ONE
ISSN: 1932-6203
Popis: The modified multiple platform method (MMPM) is a classical sleep deprivation model. It has been widely used in behavioral and brain research, due to its effects on physical and mental functions. However, different MMPM protocols can promote distinct effects in rats. Although the MMPM has been proved to induce central fatigue, the effects of different durations of subjection to the MMPM remain undetermined. This study aims to investigate the changes in behavior, N-Methyl-d-Aspartate receptor 1 (NR1) and 2A (NR2A), as well as the ultrastructural alteration in the hippocampus after different MMPM modelling, to compare the central fatigue effect induced by dynamic MMPM. Rats were randomly divided into four groups: 5-, 14- and 21- day MMPM groups, and a control group. Each MMPM group underwent a 14-hour daily MMPM modelling. After each training session, open field and elevated plus maze tests were performed. Corticosterone levels were detected by ELISA, and the hippocampal NR1 and NR2A were measured by RT-PCR and Western blot analysis. In addition, ultrastructural changes in the hippocampal cornu ammonis 1(CA1) region were determined by transmission electron microscopy (TEM). The findings showed that the 5 and 14 days of MMPM induced a high-stress state, while the 21 days of MMPM induced anxiety and degenerative alteration in the hippocampal morphology. Additionally, hippocampal NR1 and NR2A gene expression decreased in all MMPM groups, whereas the protein expression only decreased in the 21-day group. Overall, different durations of MMPM caused distinct behavioral and brain changes, and the 21 days of MMPM could induce central fatigue.
Databáze: OpenAIRE
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