The Effects of ZnO Nanoparticles in Combination with Alcohol on Biosynthetic Potential of Saccharomyces cerevisiae
| Autor: | Natalia Chiseliţa, Nadejda Efremova, Agafia Usatîi |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Saccharomyces cerevisiae carbohydrates Nanoparticle Biomass Alcohol 01 natural sciences Food processing and manufacture Industrial and Manufacturing Engineering 03 medical and health sciences chemistry.chemical_compound Biosynthesis 010608 biotechnology biomass production Protein biosynthesis saccharomyces cerevisiae Food science biology alcohol TP368-456 biology.organism_classification proteins Enzyme assay 030104 developmental biology chemistry catalase activity Catalase biology.protein nanoparticles β-glucans Food Science |
| Zdroj: | Acta Universitatis Cibiniensis. Series E: Food Technology, Vol 21, Iss 2, Pp 19-24 (2017) |
| ISSN: | 2344-150X |
| Popis: | This paper reports about experimental results concerning the influence of 30 nm ZnO nanoparticles on biomass, carbohydrates, β-glucans, proteins accumulation and catalase enzyme activity at Saccharomyces cerevisiae CNMN-Y-20 yeast strain exposed to alcohol action. Alcohol in concentrations of 2%, 5% and 10% added to culture medium has been reported to stimulate β-glucans biosynthesis and to inhibit protein synthesis. Low biomass production, with 71% less that control, was detected in the experiments with 10% alcohol. ZnO nanoparticles in combination with alcohol do not offer sufficient protection for the proteins biosynthesis, but efficiently protect the carbohydrates and β-glucans biosynthetic processes, which contents in the biomass are with 16.6% and 19.9% higher than control, respectively. The maximum value of β-glucans content was established in case of cultivation of selected yeast strain on YPD medium supplemented with 5 mg/L nanoparticles ZnO and 2% alcohol. The obtained results allowed the elaboration of new procedure for directed synthesis of β-glucans that contributed to an increase of this component with 30.7%, compared to control. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|