| Autor: |
Cholil Yun, Xiaochao Ji, Yuwen Chen, Zhuowen Zhao, Yuan Gao, Lin Gu, Danqi She, Ilbong Ri, Wenjie Wang, Huimei Wang |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
International journal of biological macromolecules. 227 |
| ISSN: |
1879-0003 |
| Popis: |
Scutellaria baicalensis is a medicinal plant possessing abundant bioactive polysaccharides. This study aimed to optimize the ultrasound-assisted enzymatic extraction of S. baicalensis root polysaccharide (SRP) and investigate its hypoglycemic and immunomodulatory activities. The optimal extraction conditions found in this study were as follows: cellulase concentration 165.6 U/mL, temperature 57.3 °C, liquid-solid ratio 44.8 mL/g, time 50 min, and ultrasonic power 225 W; with the yield reached up to 12.27 %. The ion exchange and gel filtration chromatographies were used to obtain a purified SRP. The carbohydrate content of SRP was 85.09 %, with a relatively high content of uronic acids (11.27 %). The SRP had a molecular weight of 89.7 kDa and was composed of eight monosaccharides. The inhibitory activity of SRP against α-amylase and α-glucosidase was determined. It was revealed that SRP could effectively inhibit these two enzymes with IC |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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