Determination of ciprofloxacin in human plasma using high-performance liquid chromatography coupled with fluorescence detection: Application to a population pharmacokinetics study in children with severe malnutrition
| Autor: | Gilbert Kokwaro, Alex Muturi, Nahashon Thuo, Simon N. Muchohi, Japhet Karisa, Kathryn Maitland |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Clinical Biochemistry
010501 environmental sciences 01 natural sciences Biochemistry High-performance liquid chromatography Child Nutrition Disorders Sensitivity and Specificity Article Analytical Chemistry Plasma Sarafloxacin Drug Stability Ciprofloxacin Validation medicine Protein precipitation Humans Least-Squares Analysis Child Chromatography High Pressure Liquid 0105 earth and related environmental sciences Antibacterial agent Detection limit Chromatography Elution Chemistry 010401 analytical chemistry Malnutrition Reproducibility of Results Cell Biology General Medicine 0104 chemical sciences Spectrometry Fluorescence HPLC fluorescence detection Quantitative analysis (chemistry) medicine.drug |
| Zdroj: | Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences |
| Popis: | Article published in Journal of Chromatography B Clinical pharmacokinetic studies of ciprofloxacin require accurate and precise measurement of plasma drug concentrations. We describe a rapid, selective and sensitive HPLC method coupled with fluorescence detection for determination of ciprofloxacin in human plasma. Internal standard (IS; sarafloxacin) was added to plasma aliquots (200uL) prior to protein precipitation with acetonitrile. Ciprofloxacin and IS were eluted on a Synergi Max-RP analytical column (150mm×4.6mm i.d., 5um particle size) maintained at 40 ◦C. The mobile phase comprised a mixture of aqueous orthophosphoric acid (0.025 M)/methanol/acetonitrile (75/13/12%, v/v/v); the pH was adjusted to 3.0 with triethylamine. A fluorescence detector (excitation/emission wavelength of 278/450 nm) was used. Retention times for ciprofloxacin and IS were approximately 3.6 and 7.0 min, respectively. Calibration curves of ciprofloxacin were linear over the concentration range of 0.02–4ug/mL, with correlation coefficients (r2)≥0.998. Intraand inter-assay relative standard deviations (SD) were |
| Databáze: | OpenAIRE |
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