Effect of Metabolic Stress on the Expression of mTOR Kinase in Mouse Liver Cells
| Autor: | E. A. Martinova, M. M. G. Gapparov, A. V. Vasiliev |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
Protein Serine-Threonine Kinases General Biochemistry Genetics and Molecular Biology Mice Stress Physiological Internal medicine medicine Animals Metabolic Stress Protein kinase B Cells Cultured PI3K/AKT/mTOR pathway biology Kinase TOR Serine-Threonine Kinases RPTOR Intracellular Signaling Peptides and Proteins Fasting General Medicine Flow Cytometry Hsp70 Mice Inbred C57BL Endocrinology Liver Hepatic stellate cell biology.protein Female Antibody |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 148:780-784 |
| ISSN: | 1573-8221 0007-4888 |
| DOI: | 10.1007/s10517-010-0815-5 |
| Popis: | The time course of changes in mTOR kinase protein and Akt and HSP70 molecular chaperone in hepatic cells was studied in a model system of metabolic stress (48-h food deprivation with subsequent restoration of normal volume of nutrition) on C57Bl/6 mice. The fl uorescence intensity of mTOR protein stained with antibodies decreased in all liver cells during fasting. The expression of mTOR protein in different populations of liver cells was heterogeneous. The percentage of cells with initially high expression of mTOR protein decreased during 48-h food deprivation, while the percentage of cells with low mTOR expression increased. The levels of mTOR, Akt, and HSP70 returned to normal only on day 4 after normalization of nutrition. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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