The origins and regulation of tissue tension: Identification of collagen tension-fixation process in vitro
| Autor: | Vivek Mudera, Nick Wilson-Jones, Robert A. Brown, Massimo Marenzana |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Cytochalasin D
Matrix (biology) Biology Tissue Culture Techniques Transforming Growth Factor beta1 chemistry.chemical_compound Transforming Growth Factor beta Collagen network Animals Humans Regeneration Cytochalasin Process (anatomy) Fixation (histology) Tissue Engineering Tension (physics) Water Soft tissue Cell Biology Anatomy Fibroblasts Elasticity In vitro Extracellular Matrix Rats Kinetics chemistry Connective Tissue Biophysics Collagen Stress Mechanical Gels |
| Zdroj: | Experimental Cell Research. 312:423-433 |
| ISSN: | 0014-4827 |
| DOI: | 10.1016/j.yexcr.2005.11.005 |
| Popis: | The absence of a controllable in vitro model of soft tissue remodeling is a major impediment, limiting our understanding of collagen pathologies, tissue repair and engineering. Using 3D fibroblast-collagen lattice model, we have quantified changes in matrix tension and material properties following remodeling by blockade of cell-generated tension with cytochalasin D. This demonstrated a time-dependent shortening of the collagen network, progressively stabilized into a built-in tension within the matrix. This was differentially enhanced by TGFB1 and mechanical loading to give subtle control of the new, remodeled matrix material properties. Through this model, we have been able to identify the 'tension remodeling' process, by which cells control material properties in response to environmental factors. |
| Databáze: | OpenAIRE |
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