Improved enzyme-linked immunosorbent assay for the detection of orbivirus antigens by treatment with sodium dodecyl sulfate
| Autor: | John J. Oprandy |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Microbiology (medical)
chemistry.chemical_classification Orbivirus biology Sodium Dodecyl Sulfate Enzyme-Linked Immunosorbent Assay General Medicine biology.organism_classification Reoviridae Molecular biology Virus Cell Line chemistry.chemical_compound Infectious Diseases Enzyme chemistry Antigen Animals Regression Analysis Sodium dodecyl sulfate Antigens Viral |
| Zdroj: | Diagnostic microbiology and infectious disease. 7(1) |
| ISSN: | 0732-8893 |
| Popis: | Several methods have been developed for treating antigens, that reduce background or inactivate virus utilizing toxic reagents for the enzyme-linked immunosorbent assay (ELISA). In this study, orbivirus infected BHK-2 1 cells were treated with SDS for increased sensitivity in antigen detection by ELISA. The sensitivity of 1.0% SDS treated material increased eightfold. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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