Ontogeny of Rat Lung Type IV Collagenase mRNA Expression and Collagenolytic Activity during the Perinatal Period
| Autor: | Wiley Taylor, Milagros Malicdem, Uday P. Devaskar, Mary Goerke |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
medicine.medical_specialty
Cellular differentiation Gene Expression Biology Betamethasone Rats Sprague-Dawley Extracellular matrix Type IV collagen Fetus Pregnancy Internal medicine Gene expression medicine Animals Collagenases RNA Messenger Northern blot Lung Maternal-Fetal Exchange Basement membrane Molecular biology Rats medicine.anatomical_structure Endocrinology Animals Newborn Pediatrics Perinatology and Child Health Collagenase Female Collagen Developmental Biology medicine.drug |
| Zdroj: | Neonatology. 64:376-381 |
| ISSN: | 1661-7819 1661-7800 |
| DOI: | 10.1159/000244014 |
| Popis: | The extracellular matrix is important in the cellular differentiation and morphogenesis of the lung. The basement membrane (BM), an integral part of the extracellular matrix, is composed primarily of type of IV collagen. The metabolism of type IV collagenase is important in remodeling of BM that occurs during growth. We examined the ontogeny of rat lung type IV collagenase mRNA expression, type IV collagenolytic activity and type IV collagen content during the perinatal period. In addition, the effect of prenatal glucocorticoid (GC) treatment on fetal lung type IV collagenase mRNA expression and type IV collagenolytic activity was studied. Lung polyadenylated RNA was extracted and subjected to Northern blot analysis and laser densitometry after hybridization with human type IV collagenase (approximately 72 kD) and rat actin cDNA probe. Type IV collagenolytic activity and type IV collagen concentration were quantitated by an enzymatic and a radioimmunoassay, respectively. While lung type IV collagenase mRNA to alpha-actin ratio and type IV collagenolytic activity were highest prior to birth (21-day fetus), the lung type IV collagen concentration was lowest at this time. Prenatal GC treatment did not influence type IV collagenase mRNA expression or the collagenolytic activity. A role for fetal lung type IV collagenase in preparation for the neonatal pulmonary vascular and/or alveolar adaptation is proposed. |
| Databáze: | OpenAIRE |
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