Phage-AgNPs complex as SERS probe for U937 cell identification
| Autor: | Sebastiano Trusso, Caterina Musolino, Salvatore P.P. Guglielmino, Fortunato Neri, Federica Calabrese, Maria Puliafico, Enza Fazio, Alessandro Allegra, Domenico Franco, Germana Lentini, Marco Sebastiano Nicolò, Laura M. De Plano, S. Carnazza |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Cell identification
Phage display Silver Biomedical Engineering Biophysics Metal Nanoparticles Molecular Probe Techniques Spectrum Analysis Raman Sensitivity and Specificity Silver nanoparticle Bacteriophage Peptide Library Electrochemistry Biomarkers Tumor Humans biology SERS Minimal residual disease Reproducibility of Results General Medicine Neoplasms Experimental U937 Cells Surface-enhanced Raman spectroscopy Surface Plasmon Resonance biology.organism_classification U937 cells Molecular biology In vitro Biochemistry Cancer cell Biosensor Biotechnology |
| Zdroj: | Biosensors & bioelectronics 74 (2015): 398–405. doi:10.1016/j.bios.2015.05.073 info:cnr-pdr/source/autori:Lentini, Germana; Fazio, Enza; Calabrese, Federica; De Plano, Laura M.; Puliafico, Maria; Franco, Domenico; Nicolo, Marco S.; Carnazza, Santina; Trusso, Sebastiano; Allegra, Alessandro; Neri, Fortunato; Musolino, Caterina; Guglielmino, Salvatore P. P./titolo:Phage-AgNPs complex as SERS probe for U937 cell identification/doi:10.1016%2Fj.bios.2015.05.073/rivista:Biosensors & bioelectronics/anno:2015/pagina_da:398/pagina_a:405/intervallo_pagine:398–405/volume:74 |
| DOI: | 10.1016/j.bios.2015.05.073 |
| Popis: | The early diagnosis of malignancy is the most critical factor for patient survival and the treatment of cancer. In particular, leukemic cells are highly heterogeneous, and there is a need to develop new rapid and accurate detection systems for early diagnosis and monitoring of minimal residual disease. This study reports the utilization of molecular networks consisting of entire bacteriophage structure, displaying specific peptides, directly assembled with silver nanoparticles as a new Surface Enhanced Raman Spectroscopy (SERS) probe for U937 cells identification in vitro. A 9-mer pVIII M13 phage display library is screened against U937 to identify peptides that selectively recognize these cells. Then, phage clone is assembled with silver nanoparticles and the resulting network is used to obtain a SERS signal on cell-type specific molecular targets. The proposed strategy could be a very sensitive tool for the design of biosensors for highly specific and selective identification of hematological cancer cells and for detection of minimal residual disease in a significant proportion of human blood malignancy. (C) 2015 Elsevier B.V. All rights reserved. |
| Databáze: | OpenAIRE |
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