| Autor: |
Yu-Han Chen, Jiahong Dong, Wan-Chu Chien, Kuanyu Zheng, Wu, Kuo, Shyi-Dong Yeh, Sun, Jing-Hua, Yun-Chi Wang, Tsung-Chi Chen |
| Rok vydání: |
2016 |
| Předmět: |
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| DOI: |
10.6084/m9.figshare.c.3627365_d2 |
| Popis: |
Comparison of the serological relationship of Calla lily chlorotic spot virus (CCSV), Tomato zonate spot virus (TZSV) and Tomato necrotic spot associated virus (TNSaV). The previously reported monoclonal antibodies MAb-CCSV-NP [24] (A and B) and MAb-WNSs [25] (C and D) were used to react with the crude leaf extracts of Nicotiana benthamiana plants separately infected with CCSV, TZSV, TNSaV or Watermelon silver mottle virus (WSMoV) in indirect enzyme-linked immunosorbent assay (A and C) and immunoblotting (B and D). The crude extract of a healthy N. benthamiana leaf (H) was used as the negative control. The plant ribulose bisphosphate carboxylase/oxygenase (rubisco) of Ponceau S staining is shown under immunoblotting to indicate the loading quantity. MAb-CCSV-NP was used at a 10â 4 dilution. MAb-WNSs was used at a 10â 3 dilution. (DOCX 124 kb) |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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