A Golden Gate Modular Cloning Toolbox for Plants
| Autor: | Sylvestre Marillonnet, Nicola J. Patron, Tim-Martin Ehnert, Jonathan D. G. Jones, Ramona Gruetzner, Stefan Werner, Mark Youles, Carola Engler |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Golden Gate Cloning
Genetic Vectors Biomedical Engineering Cloning vector Nicotiana benthamiana Computational biology Molecular cloning Biology Biochemistry Genetics and Molecular Biology (miscellaneous) Synthetic biology Tobacco Cloning Molecular Selectable marker Models Genetic Cloning (programming) business.industry fungi food and beverages General Medicine biology.organism_classification Biotechnology Transformation (genetics) Agrobacterium tumefaciens Synthetic Biology Genetic Engineering business |
| Zdroj: | ACS Synthetic Biology. 3:839-843 |
| ISSN: | 2161-5063 |
| Popis: | Plant Synthetic Biology requires robust and efficient methods for assembling multigene constructs. Golden Gate cloning provides a precision module-based cloning technique for facile assembly of multiple genes in one construct. We present here a versatile resource for plant biologists comprising a set of cloning vectors and 96 standardized parts to enable Golden Gate construction of multigene constructs for plant transformation. Parts include promoters, untranslated sequences, reporters, antigenic tags, localization signals, selectable markers, and terminators. The comparative performance of parts in the model plant Nicotiana benthamiana is discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|