Molecular characterization of neuropeptide F from the eastern subterranean termite Reticulitermes flavipes (Kollar) (Isoptera: Rhinotermitidae)
Autor: | Jan Pohl, Victoria TeBrugge, Joe W. Crim, Andrew B. Nuss, Brian T. Forschler, Mark R. Brown |
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Rok vydání: | 2010 |
Předmět: |
Physiology
media_common.quotation_subject Molecular Sequence Data Radioimmunoassay Zoology Grasshoppers Isoptera Insect Biochemistry Cellular and Molecular Neuroscience Reticulitermes Endocrinology Phylogenetics Animals Amino Acid Sequence Peptide sequence Chromatography High Pressure Liquid Phylogeny media_common Base Sequence biology Ecology Neuropeptides Midgut biology.organism_classification Gastrointestinal Tract Eastern subterranean termite Helicoverpa zea Sequence Alignment Rhinotermitidae |
Zdroj: | Peptides. 31:419-428 |
ISSN: | 0196-9781 |
DOI: | 10.1016/j.peptides.2009.09.001 |
Popis: | Neuropeptide F (NPF)-like immunoreactivity was previously found to be abundant in the eastern subterranean termite, Reticulitermes flavipes. Purification of the NPF from a whole body extract of worker termites was accomplished in the current study by HPLC and heterologous radioimmunoassay for an NPF-related peptide, Helicoverpa zea Midgut Peptide-I. A partial amino acid sequence allowed determination of the corresponding cDNA that encoded an open reading frame deduced for authentic R. flavipes NPF (Ref NPF): KPSDPEQLADTLKYLEELDRFYSQVARPRFa. Effects of synthetic NPFs on muscle contractions were investigated for isolated foreguts and hindguts of workers, with Drm NPF inhibiting spontaneous contractions of hindguts. Phylogenetic analysis of invertebrate NPF sequences reveals two separate groupings, with Ref NPF occurring within a clade composed exclusively of arthropods. |
Databáze: | OpenAIRE |
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