Discovery of α-Glucosidase Inhibitors from Marine Microorganisms: Optimization of Culture Conditions and Medium Composition
| Autor: | Nguyen Thi Hien Trang, Kit Wayne Chew, Pau Loke Show, Nguyen Thi Trung, Do Thi Tuyen, Nguyen Thi Thao, Doris Ying Ying Tang, Nguyen Thi Thuy Linh, Nguyen Tien Cuong, Le Thanh Hoang, Hoang Thi Yen |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Aquatic Organisms Microorganism Cell Culture Techniques Bioengineering Fungus 01 natural sciences Applied Microbiology and Biotechnology Biochemistry Aeromonadaceae 03 medical and health sciences Marine bacteriophage 010608 biotechnology Drug Discovery medicine Humans Glycoside Hydrolase Inhibitors Food science Molecular Biology IC50 030304 developmental biology Acarbose 0303 health sciences biology Chemistry alpha-Glucosidases biology.organism_classification Culture Media Microbial population biology Diabetes Mellitus Type 2 Fermentation Composition (visual arts) Biotechnology medicine.drug |
| Zdroj: | Molecular biotechnology. 63(11) |
| ISSN: | 1559-0305 |
| Popis: | Various studies showed that the suppression of α-glucosidase activity can impede the glucose absorption in our body, and therefore, it can be used to treat type 2 diabetes. Hence, the compounds with anti-α-glucosidase have gained considerable attention because of their potential application in diabetes treatment. In previous literature studies, these anti-α-glucosidase compounds were extracted from plants and fungus. Less studies are being conducted to identify the anti-α-glucosidase compounds in the microbial community. In this study, 23 marine bacterial strains were screened for their potential to suppress the α-glucosidase activity. The highest inhibitory activity was exhibited by isolated L06 which was identified as Oceanimonas smirnovii EBL6. The cultivation conditions, such as temperature and pH, were optimized to increase the production of α-glucosidase inhibitors by Oceanimonas smirnovii EBL6 strain. The result findings showed that the highest yield of α-glucosidase inhibitors can be obtained at the culture time of 120 h, fermentation temperature of 30 °C, and pH 4.6. Under these conditions, the inhibitory activity of α-glucosidase can reach 81%. The IC50 of n-butanol extract was 13.89 μg/ml, while standard acarbose was 31.16 μg/ml. Overall, these findings suggest that Oceanimonas smirnovii produces α-glucosidase inhibitors and could been applied in the biochemical and medicinal fields in the future. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink