High level production of itaconic acid at low pH by Ustilago maydis with fed-batch fermentation
| Autor: | Emrah Nikerel, Nick Wierck, Johanna Becker, Mustafa Türker, Emine Bezirci, Hatice Taşpınar Demir, Hamed Hosseinpour Tehrani |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Batch fermentation Nitrogen Ustilago Bioengineering 01 natural sciences Phosphates Substrate Specificity Fungal Proteins Industrial Microbiology chemistry.chemical_compound Bioreactors ddc:690 010608 biotechnology Biomass yield Extracellular Biomass Food science Itaconic acid biology 010405 organic chemistry Chemistry Basidiomycota Temperature Succinates General Medicine Carbon Dioxide Hydrogen-Ion Concentration biology.organism_classification Carbon 0104 chemical sciences Glucose Yield (chemistry) Fermentation Industrial and production engineering Biotechnology |
| Zdroj: | Bioprocess and biosystems engineering 44(4), 749-758 (2021). doi:10.1007/s00449-020-02483-6 |
| ISSN: | 1615-7605 1615-7591 |
| Popis: | The metabolically engineered plant pathogen Ustilago maydis MB215 Delta cyp3 P(etef)ria1 has been cultivated to produce more than 80 g/L itaconate in 16 L scale pH and temperature controlled fermentation, in fed-batch mode with two successive feedings. The effect of pH as well as successive rounds of feeding has been quantified via elemental balances. Volumetric itaconic acid productivity gradually decreased with successive glucose feedings with increasing itaconic titers, with nearly constant product yield. Extracellular pH was decreased from 6 down to 3.5 and the fermentation was characterized in specific uptake, production, and growth rates. Notable is that the biomass composition changes significantly from growth phase to itaconic acid production phase, carbon content increases from 42% to around 62%. Despite the gradual decrease in itaconic acid levels with decreasing pH (nearly 50% decrease in itaconic acid at pH 3.5, compared to pH 6), significant itaconate production is still observed at pH 4 (around 63 g/L). Biomass yield remained nearly constant until pH 4. Taken together, these results strongly illustrate the potential of engineered Ustilago maydis in itaconate production at commercial levels. |
| Databáze: | OpenAIRE |
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