Post-mitotic, differentiated myotubes efficiently produce retroviral vector from hybrid adeno-retrovirus templates
| Autor: | Takis Athanasopoulos, Marita Pohlschmidt, George Dickson, Michael L. Roberts, Ghislaine Duisit, François-Loïc Cosset |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
viruses
Genetic enhancement Genetic Vectors Green Fluorescent Proteins Muscle Fibers Skeletal Gene Expression Biology Recombinant virus medicine.disease_cause Antiviral Agents Adenoviridae Cell Line Viral vector Mice Retrovirus Aphidicolin Genetics medicine Animals Humans Vector (molecular biology) Molecular Biology Mitosis Myogenesis Cell Differentiation 3T3 Cells Genetic Therapy biology.organism_classification Virology Muscular Dystrophy Duchenne Luminescent Proteins Retroviridae Microscopy Fluorescence Molecular Medicine Genetic Engineering |
| Zdroj: | Gene Therapy. 8:1580-1586 |
| ISSN: | 1476-5462 0969-7128 |
| Popis: | We have examined the ability of proliferating myoblasts and post-mitotic, differentiated myotubes to produce retroviral vector using hybrid adeno-retroviral vectors as templates. We show that production of retroviral vector from myoblasts peaks 48 h after adenoviral infection at 4.8 x 10(4) cfu/ml and is scarcely detectable by 96 h. Both fully and partially differentiated myotubes were able to generate a sustained increase in the levels of retroviral vector compared with myoblasts peaking 48 h at 1.4 x 10(5) cfu/ml and 1.8 x 10(5) cfu/ml, respectively. Addition of the cell cycle inhibitor aphidicolin (5 microg/ml) had no effect on the production of retroviral vector from fully differentiated myotubes, but resulted in an 80% increase in vector production from partially differentiated myotubes. Thus indicating that retroviral vector production is more efficient in post-mitotic myotubes and is independent of muscle cell cycle progression. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|